In situ visualization of DNA double-strand break repair in human fibroblasts

被引：409

作者：

Nelms, BE

Maser, RS

MacKay, JF

Lagally, MG

Petrini, JHJ ^{[1
]}

机构：

[1] Univ Wisconsin, Sch Med, Genet Lab, Madison, WI 53706 USA

[2] Univ Wisconsin, Sch Med, Dept Med Phys, Madison, WI 53706 USA

[3] Univ Wisconsin, Sch Med, Dept Mat Sci & Engn, Madison, WI 53706 USA

来源：

SCIENCE | 1998年 / 280卷 / 5363期

关键词：

D O I：

10.1126/science.280.5363.590

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A method was developed to examine DNA repair within the intact cell. Ultrasoft x-rays were used to induce DNA double-strand breaks (DSBs) in defined subnuclear volumes of human fibroblasts and DNA repair was visualized at those sites. The DSBs remained in a fixed position during the initial stages of DNA repair, and the DSB repair protein hMre11 migrated to the sites of damage within 30 minutes. In contrast, hRad51, a human RecA homolog, did not localize at sites of DNA damage, a finding consistent with the distinct roles of these proteins in DNA repair.

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收藏

页码：590 / 592

页数：3

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