Quantitative proteomic assessment of very early cellular signaling events

被引：92
作者：
Dengjel, Joern
Akimov, Vyacheslav
Olsen, Jesper V.
Bunkenborg, Jakob
Mann, Matthias
Blagoev, Blagoy
Andersen, Jens S. [1 ]
机构：
[1] Univ So Denmark, Ctr Expt Bioinformat, Dept Biochem & Mol Biol, DK-5230 Odense, Denmark
[2] Max Planck Inst Biochem, Dept Proteom & Signal Transduct, D-82152 Martinsried, Germany
来源：
NATURE BIOTECHNOLOGY | 2007年 / 25卷 / 05期
关键词：
D O I：
10.1038/nbt1301
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Technical limitations have prevented proteomic analyses of events occurring less than 30 s after signal initiation. We developed an automated, continuous quench-flow system allowing quantitative proteomic assessment of very early cellular signaling events (qPACE) with a time resolution of 1 s. Using this technique, we determined that autophosphorylation of the epidermal growth factor receptor occurs within 1 s after ligand stimulation and is followed rapidly by phosphorylation of the downstream signaling intermediates Src homologous and collagen-like protein and phospholipase C gamma 1.
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页码：566 / 568
页数：3
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