Detection of bovine herpesvirus 1 (BHV-1) genomic sequences in bovine semen inoculated with BHV-1 by polymerase chain reaction

被引：0
|
作者：
Kataria, RS
Tiwari, AK
Gupta, PK
Mehrotra, ML
Rai, A
Bandyopadhyay, SK [1 ]
机构：
[1] Indian Vet Res Inst, Natl Biotechnol Ctr, Bareilly 243122, Uttar Pradesh, India
[2] Indian Vet Res Inst, Ctr Anim Dis Res & Diag, Bareilly 243122, Uttar Pradesh, India
[3] Indian Vet Res Inst, Standardizat Div, Bareilly 243122, Uttar Pradesh, India
来源：
ACTA VIROLOGICA | 1997年 / 41卷 / 06期
关键词：
bovine herpesvirus 1; PCR sensitivity; semen;
D O I：
暂无
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Polymerase chain reaction (PCR) technique was applied to detect BHV-1 in bovine semen inoculated with BHV-1. The technique was found to be 10(6) times more sensitive than a non-isotopic dot-blot hybridization method in detecting viral genomic DNA. Of the three primer pairs used, the one chosen from glycoprotein gC appeared to be most sensitive as it could detect up to 0.01 TCID50 of BHV-1 in the semen. The technique could be useful in screening breeding bulls or samples of frozen semen prior to use in artificial insemination.
引用
收藏
页码：311 / 315
页数：5
相关论文
共 50 条
  • [1] RAPID DETECTION OF BOVINE HERPESVIRUS-1 (BHV-1) USING THE POLYMERASE CHAIN-REACTION
    VILCEK, S
    NETTLETON, PF
    HERRING, JA
    HERRING, AJ
    VETERINARY MICROBIOLOGY, 1994, 42 (01) : 53 - 64
  • [2] Detection of different Brazilian strains of the bovine herpesvirus-1 (BHV-1) by polymerase chain reaction
    Cândido, AL
    Martins, AS
    Barros, PB
    Resende, M
    ARQUIVO BRASILEIRO DE MEDICINA VETERINARIA E ZOOTECNIA, 1999, 51 (03) : 241 - 244
  • [3] DETECTION OF THE BOVINE HERPESVIRUS-1 (BHV-1) GENOME BY PCR
    VILCEK, S
    JOURNAL OF VIROLOGICAL METHODS, 1993, 41 (02) : 245 - 247
  • [4] DETECTION OF BOVINE HERPESVIRUS 1 (BHV-1) INFECTION IN SEMEN OF INDIAN BREEDING BULLS BY POLYMERASE CHAIN REACTION AND ITS CHARACTERIZATION BY DNA SEQUENCING
    Lata, Jain
    Kanani, A. N.
    Purohit, J. H.
    Joshi, C. G.
    Rank, D. N.
    Kumar, Vinay
    Jain, V. K.
    BUFFALO BULLETIN, 2009, 28 (02): : 76 - 84
  • [5] Development of a nested PCR for detection of Bovine herpesvirus-1 (BHV-1) in bovine nasal secretion and semen
    Saepulloh, Muharam
    Adjid, R. M. Abdul
    Wibawan, I. Wayan T.
    Darminto
    JURNAL ILMU TERNAK DAN VETERINER, 2008, 13 (02) : 155 - 164
  • [6] DETECTION OF BOVINE HERPESVIRUS 1 (BHV-1) INFECTION IN SEMEN OF INDIAN BREEDING BULLS BY POLYMERASE CHAIN REACTION AND ITS CHARACTERIZATION BY DNA SEQUENCING
    Lata, Jain
    Kanani, A. N.
    Purohit, J. H.
    Joshi, C. G.
    Rank, D. N.
    Kumar, Vinay
    Jain, V. K.
    BUFFALO BULLETIN, 2010, 29 (01): : 21 - 30
  • [7] RISK OF TRANSMISSION OF BOVINE HERPESVIRUS ( BHV-1) BY INFECTED SEMEN TO RECIPIENTS AND EMBRYOS
    Bielanski, A.
    Lalonde, A.
    Algire, J.
    REPRODUCTION FERTILITY AND DEVELOPMENT, 2009, 21 (01) : 173 - 173
  • [8] BOVINE HERPESVIRUS-1 (BHV-1) - BIOLOGY, PATHOGENESIS, AND CONTROL
    TIKOO, SK
    CAMPOS, M
    BABIUK, LA
    ADVANCES IN VIRUS RESEARCH, VOL 45, 1995, 45 : 191 - 223
  • [9] Bovine Herpesvirus Type 1 (BHV-1) Prevalence in Dairy Cattle
    Iscan, Uzeyir Taha
    Duman, Rustem
    JOURNAL OF ANIMAL AND VETERINARY ADVANCES, 2011, 10 (12): : 1523 - 1525
  • [10] PROTEOLYTIC CLEAVAGE OF BOVINE HERPESVIRUS-1 (BHV-1) GLYCOPROTEIN GB IS NOT NECESSARY FOR ITS FUNCTION IN BHV-1 OR PSEUDORABIES VIRUS
    KOPP, A
    BLEWETT, E
    MISRA, V
    METTENLEITER, TC
    JOURNAL OF VIROLOGY, 1994, 68 (03) : 1667 - 1674
12345