Acetylation and nitrosation of ciprofloxacin by environmental strains of mycobacteria

被引：22

作者：

Adjei, Michael D. ^{[1
]}

Heinze, Thomas M. ^{[1
]}

Deck, Joanna ^{[1
]}

Freeman, James P. ^{[1
]}

Williams, Anna J. ^{[1
]}

Sutherland, John B. ^{[1
]}

机构：

[1] US FDA, Natl Ctr Toxicol Res, Jefferson, AR 72079 USA

来源：

CANADIAN JOURNAL OF MICROBIOLOGY | 2007年 / 53卷 / 01期

关键词：

acetylation; ciprofloxacin; fluoroquinolones; Mycobacterium; nitrosation;

D O I：

10.1139/W06-101

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To determine the ability of environmental bacteria to metabolize the frequently prescribed fluoroquinolone drug ciprofloxacin, eight Mycobacterium spp. cultures were grown for 4 days in a medium containing sorbitol and yeast extract with 100 mg. L-1 ciprofloxacin. After the cultures had been centrifuged and the supernatants extracted with ethyl acetate, two metabolites were purified by using high-performance liquid chromatography. They were identified with liquid chromatography/electro spray ionization mass spectrometry and proton nuclear magnetic resonance spectroscopy. Ciprofloxacin was transformed to both N-acetylciprofloxacin (2.5%-5.5% of the total peak area at 280 nm) and N-nitrosociprofloxacin (6.0%-8.0% of the peak area) by Mycobacterium gilvum PYR-GCK and Mycobacterium sp. PYR100 but it was transformed only to N-acetylciprofloxacin by Mycobacterium frederiksbergense FAn9, M. gilvum ATCC 43909, M. gilvum BB 1, Mycobacterium smegmatis me 2 155, Mycobacterium sp. 7E1B1W, and Mycobacterium sp. RJGII-135. The results suggest that biotransformation may serve as a ciprofloxacin resistance mechanism for these bacteria.

引用

页码：144 / 147

页数：4

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