Differential expression profile of plasma exosomal microRNAs in acute type A aortic dissection with acute lung injury

被引：11

作者：

Zhang, Chiyuan ^{[1
,3
]}

Bai, Hui ^{[1
]}

Zhang, Lei ^{[1
]}

Zhang, Yanfeng ^{[1
]}

Chen, Xuliang ^{[1
]}

Shi, Ruizheng ^{[2
]}

Zhang, Guogang ^{[3
]}

Xu, Qian ^{[1
]}

Lin, Guoqiang ^{[1
]}

机构：

[1] Cent South Univ, Xiangya Hosp, Dept Cardiovasc Surg, Xiangya Rd 87, Changsha 410008, Hunan, Peoples R China

[2] Cent South Univ, Xiangya Hosp, Dept Cardiovasc Med, Xiangya Rd 87, Changsha, Hunan, Peoples R China

[3] Cent South Univ, Dept Cardiovasc Med, Xiangya Hosp 3, Tongzipo Rd 138, Changsha, Hunan, Peoples R China

来源：

SCIENTIFIC REPORTS | 2022年 / 12卷 / 01期

关键词：

PREOPERATIVE HYPOXEMIA; ENDOTHELIAL-CELLS; ANGIOTENSIN-II; INFLAMMATION; APOPTOSIS; PROTECTS; MODULATE; TARGETS; SEPSIS; CANCER;

D O I：

10.1038/s41598-022-15859-3

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

MicroRNAs (miRNAs) packaged into exosomes mediate cell communication and contribute to the pathogenesis of acute type A aortic dissection (ATAAD) with acute lung injury (ALI). The expression profile of plasma exosomal miRNAs in ATAAD patients with ALI hasn't been identified. We performed a miRNA-sequencing to analyze the differentially expressed miRNAs (DE-miRNAs) of circulating exosomes in ATAAD patients with ALI compared to patients without ALI, founding 283 specific miRNAs in two groups. We respectively selected the top 10 downregulated and upregulated DE-miRNAs for further studies. The predicted transcription factors (TFs) of these DE-miRNAs were SMAD2, SRSF1, USF1, etc. The Gene Ontology (GO) and Kyoto Encyclopedia Genes and Genomes (KEGG) analysis predicted their target genes mainly involved acute inflammatory response, cell junction, cytoskeleton, NF-kappa B signaling pathway, etc. Construction and analysis of the PPI network revealed that RHOA and INSR were considered hub genes with the highest connectivity degrees. Moreover, we confirmed two exosomal miRNAs (hsa-miR-485-5p and hsa-miR-206) by real-time quantitative polymerase chain reaction (RT-qPCR) in a validation cohort. Our study identified a plasma exosomal miRNAs signature related to ATAAD with ALI. Certain DE-miRNAs may contribute to the progression of this disease, which help us better understand the pathogenesis of ATAAD with ALI.

引用

页数：12

共 62 条

[1] Acute lung injury: The therapeutic role of Rho kinase inhibitors [J].

Abedi, Farshad ;

Hayes, A. Wallace ;

Reiter, Russel ;

Karimi, Gholamreza .

PHARMACOLOGICAL RESEARCH, 2020, 155

[2] Predicting effective microRNA target sites in mammalian mRNAs [J].

Agarwal, Vikram ;

Bell, George W. ;

Nam, Jin-Wu ;

Bartel, David P. .

ELIFE, 2015, 4

[3] Targeting WNT1-inducible signaling pathway protein 2 alters human breast cancer cell susceptibility to specific lysis through regulation of KLF-4 and miR-7 expression [J].

Akalay, I. ;

Tan, T. Z. ;

Kumar, P. ;

Janji, B. ;

Mami-Chouaib, F. ;

Charpy, C. ;

Vielh, P. ;

Larsen, A. K. ;

Thiery, J. P. ;

Sabbah, M. ;

Chouaib, S. .

ONCOGENE, 2015, 34 (17) :2261-2271

[4] What Is Nuclear Factor Kappa B (NF-κB) Doing in and to the Mitochondrion? [J].

Albensi, Benedict C. .

FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY, 2019, 7

[5] THE AMERICAN-EUROPEAN CONSENSUS CONFERENCE ON ARDS - DEFINITIONS, MECHANISMS, RELEVANT OUTCOMES, AND CLINICAL-TRIAL COORDINATION [J].

BERNARD, GR ;

ARTIGAS, A ;

BRIGHAM, KL ;

CARLET, J ;

FALKE, K ;

HUDSON, L ;

LAMY, M ;

LEGALL, JR ;

MORRIS, A ;

SPRAGG, R ;

COCHIN, B ;

LANKEN, PN ;

LEEPER, KV ;

MARINI, J ;

MURRAY, JF ;

OPPENHEIMER, L ;

PESENTI, A ;

REID, L ;

RINALDO, J ;

VILLAR, J ;

VANASBECK, BS ;

DHAINAUT, JF ;

MANCEBO, J ;

MATTHAY, M ;

MEYRICK, B ;

PAYEN, D ;

PERRET, C ;

FOWLER, AA ;

SCHALLER, MD ;

HUDSON, LD ;

HYERS, T ;

KNAUS, W ;

MATTHAY, R ;

PINSKY, M ;

BONE, RC ;

BOSKEN, C ;

JOHANSON, WG ;

LEWANDOWSKI, K ;

REPINE, J ;

RODRIGUEZROISIN, R ;

ROUSSOS, C ;

ANTONELLI, MA ;

BELOUCIF, S ;

BIHARI, D ;

BURCHARDI, H ;

LEMAIRE, F ;

MONTRAVERS, P ;

PETTY, TL ;

ROBOTHAM, J ;

ZAPOL, W .

AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 1994, 149 (03) :818-824

[6] Role of Rho GTPases in thrombin-induced lung vascular endothelial cells barrier dysfunction [J].

Birukova, AA ;

Smurova, K ;

Birukov, KG ;

Kaibuchi, K ;

Garcia, JGN ;

Verin, AD .

MICROVASCULAR RESEARCH, 2004, 67 (01) :64-77

[7]

Cao F, 2021, INFLAMMATION, V21, P1

[8] RhoA regulates lipopolysaccharide-induced lung cell injury via the Wnt/β-catenin pathway [J].

Chen, Guanhua ;

Sun, Xuedong ;

Dong, Chunxiao .

MOLECULAR MEDICINE REPORTS, 2017, 16 (06) :8501-8506

[9]

Chen HO, 2018, EUR REV MED PHARMACO, V22, P3294, DOI 10.26355/eurrev_201806_15148

[10] Suppressed nuclear factor-kappa B alleviates lipopolysaccharide-induced acute lung injury through downregulation of CXCR4 mediated by microRNA-194 [J].

Chen, Ruidong ;

Xie, Fei ;

Zhao, Jie ;

Yue, Bin .

RESPIRATORY RESEARCH, 2020, 21 (01)

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