Ca-NIR: a ratiometric near-infrared calcium probe based on a dihydroxanthene-hemicyanine fluorophore

被引：29
作者：
Collot, Mayeul [1 ]
Ponsot, Flavien [1 ]
Klymchenko, Andrey S. [1 ]
机构：
[1] Univ Strasbourg, CNRS, UMR 7213, Fac Pharm,Lab Biophoton & Pharmacol, 74 Route Rhin, F-67401 Illkirch Graffenstaden, France
来源：
CHEMICAL COMMUNICATIONS | 2017年 / 53卷 / 45期
关键词：
OFF-ON PROBE; FLUORESCENT-PROBE; IN-VIVO; NUCLEAR-ENVELOPE; LIVING CELLS; RED; ION; PH; PERMEABILITY; INDICATORS;
D O I：
10.1039/c7cc02418e
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Fluorescent calcium probes are essential tools for studying the fluctuation of calcium ions in cells. Herein, we developed Ca-NIR, the first ratiometric calcium probe emitting in the near infrared region. This probe arose from the fusion of a BAPTA chelator and a dihydroxanthene-hemicyanine fluorophore. It is efficiently excited with common 630-640 nm lasers and displays two distinct emission bands depending on the calcium concentration (K-d = similar to 8 mu M). The physicochemical and spectroscopic properties of Ca-NIR allowed for ratiometric imaging of calcium distribution in live cells.
引用
收藏
页码：6117 / 6120
页数：4
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