Increased sensitivity of malaria detection by nested polymerase chain reaction using simple sampling and DNA extraction

被引：50

作者：

Cox-Singh, J ^{[1
]}

Mahayet, S ^{[1
]}

Abdullah, MS ^{[1
]}

Singh, B ^{[1
]}

机构：

[1] Univ Sci Malaysia, Sch Med Sci, Kelantan, Malaysia

来源：

INTERNATIONAL JOURNAL FOR PARASITOLOGY | 1997年 / 27卷 / 12期

关键词：

malaria; PCR; field epidemiology; Plasmodium;

D O I：

10.1016/S0020-7519(97)00147-1

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Malaria remains a disease of underdeveloped and remote regions of the world. The application of polymerase chain reaction (PCR) technology to malaria epidemiology has the potential for increasing our knowledge and understanding of this disease. In order to study malaria in all geographical locations it is important that specimen collection and DNA extraction for PCR be kept simple. Here we report a method for extracting DNA from dried blood spots on filter paper which is capable of detecting one Plasmodium falciparum and two Plasmodium vivax parasites/mu l of whole blood by nested PCR without compromising the simplicity of specimen collection or DNA extraction. (C) 1997 Australian Society for Parasitology. Published by Elsevier Science Ltd.

引用

页码：1575 / 1577

页数：3

共 50 条

[41] Detection of bovine foetal DNA from amniotic fluid using the polymerase chain reaction
Peippo, J
Bredbacka, P
AGRICULTURAL AND FOOD SCIENCE IN FINLAND, 1996, 5 (05): : 541 - 546
[42] Detection of Mycobacterium tuberculosis complex by nested polymerase chain reaction in pulmonary and extrapulmonary specimens
da Cruz Furini, Adriana Antonia
Paro Pedro, Heloisa da Silveira
Rodrigues, Jean Francisco
Lapa Montenegro, Lilian Maria
Dantas Machado, Ricardo Luiz
Franco, Celia
Schindler, Haiana Charifker
Foschiani Dias Batista, Ida Maria
Baptista Rossit, Andrea Regina
JORNAL BRASILEIRO DE PNEUMOLOGIA, 2013, 39 (06) : 711 - 718
[43] Multiplex polymerase chain reaction for detection of bacteremia during dental extraction
Azad, Azita
Modaresi, Farzan
Zahed, Maryam
Zarei, Mahshid
Ranjbaran, Alireza
Jahrom, Zahra Kargar
JOURNAL OF INVESTIGATIVE AND CLINICAL DENTISTRY, 2019, 10 (04) : e12425
[44] Comparison of a nested polymerase chain reaction - restriction fragment length polymorphism method, the PATH antigen detection method, and microscopy for the detection and identification of malaria parasites
Parkes, R
Lo, T
Wong, Q
Isaac-Renton, J
Byrne, SK
CANADIAN JOURNAL OF MICROBIOLOGY, 2001, 47 (10) : 903 - 907
[45] Comparison of Nested Polymerase Chain Reaction and Real-Time Polymerase Chain Reaction with Parasitological Methods for Detection of Strongyloides stercoralis in Human Fecal Samples
Sharifdini, Meysam
Mirhendi, Hossein
Ashrafi, Keyhan
Hosseini, Mostafa
Mohebali, Mehdi
Khodadadi, Hossein
Kia, Eshrat Beigom
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2015, 93 (06) : 1285 - 1291
[46] Rapid detection of viable bacteria by nested polymerase chain reaction via long DNA amplification after ethidium monoazide treatment
Soejima, Takashi
Schlitt-Dittrich, Frank
Yoshida, Shin-ichi
ANALYTICAL BIOCHEMISTRY, 2011, 418 (02) : 286 - 294
[47] NESTED POLYMERASE CHAIN-REACTION FOR DETECTION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 DNA IN CLINICAL SPECIMENS
ZAZZI, M
ROMANO, L
BRASINI, A
VALENSIN, PE
JOURNAL OF MEDICAL VIROLOGY, 1992, 38 (03) : 172 - 174
[48] ADAPTATION OF A SENSITIVE DNA EXTRACTION METHOD FOR DETECTION OF ENTAMOEBA HISTOLYTICA BY REAL-TIME POLYMERASE CHAIN REACTION
Pinar, Ahmet
Akyon, Yakut
Alp, Alpaslan
Erguven, Sibel
MIKROBIYOLOJI BULTENI, 2010, 44 (03): : 453 - 459
[49] SYBR-GREEN-I DNA STAINING INCREASES THE DETECTION SENSITIVITY OF VIRUSES BY POLYMERASE CHAIN-REACTION
KARLSEN, F
STEEN, HB
NESLAND, JM
JOURNAL OF VIROLOGICAL METHODS, 1995, 55 (01) : 153 - 156
[50] Evaluation of DNA extraction methods for the detection of Shiga toxin producing Escherichia coli in food by polymerase chain reaction
McMahon, Tanis
Abdelmesih, Mariam
Gill, Alexander
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2023, 404

← 1 2 3 4 5 →