Imaging of eosin-stained brain section using two-photon excitation fluorescence microscopy

被引：2

作者：

Hou, Guozhong ^{[1
,2
]}

Dong, Zhiwei ^{[2
]}

Zhang, Sheng ^{[3
]}

Bai, Qiqi ^{[4
]}

Liu, Shuo ^{[1
]}

Xia, Yuanqin ^{[1
,2
]}

机构：

[1] Hebei Univ Technol, Ctr Adv Laser Technol, Tianjin, Peoples R China

[2] Harbin Inst Technol, Natl Key Lab Sci & Technol Tunable Laser, Harbin, Peoples R China

[3] Harbin Inst Technol, Dept Phys, Harbin, Peoples R China

[4] Hebei Univ Technol, Sch Sci, Tianjin, Peoples R China

来源：

OPTICAL ENGINEERING | 2022年 / 61卷 / 07期

基金：

中国国家自然科学基金;

关键词：

two-photon excitation fluorescence microscopy; eosin stain; eosin-stained mouse brain section; z-axis scanning; three-dimensional reconstruction; DEEP TISSUE; HIGH-SPEED; 2ND-HARMONIC GENERATION; HEMATOXYLIN; DYNAMICS; DEPTH; MR;

D O I：

10.1117/1.OE.61.7.073103

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

A two-photon excitation fluorescence (TPEF) microscopy system under broadband excitation of femtosecond pulses is built in this study. The TPEF spectrum of an eosin stain is recorded, and the TPEF microscopy system is equipped with a suitable detection window matching the TPEF spectrum of the eosin stain. TPEF microscopy is demonstrated using a solid eosin sample and eosin-stained mouse brain sections through detecting the TPEF signal from eosin staining. Three-dimensional (3D) reconstruction of the mouse brain section across its thickness is realized with several TPEF imaging frames acquired via z-axis scanning with a step size of 1 mu m. Two-dimensional TPEF imaging frames and 3D reconstruction of the brain section demonstrated high performance of TPEF microscopy in the observation of eosin-stained biological sections. (c) 2022 Society of Photo-Optical Instrumentation Engineers (SPIE)

引用

页数：10

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