Sheltering Behavior and Locomotor Activity in 11 Genetically Diverse Common Inbred Mouse Strains Using Home-Cage Monitoring

被引：69

作者：

Loos, Maarten ^{[1
,2
]}

Koopmans, Bastijn ^{[1
]}

Aarts, Emmeke ^{[3
]}

Maroteaux, Gregoire ^{[3
]}

van der Sluis, Sophie ^{[3
,4
]}

Verhage, Matthijs ^{[3
,5
]}

Smit, August B. ^{[2
]}

机构：

[1] Syl Synaptol BV, Amsterdam, Netherlands

[2] Vrije Univ Amsterdam, Dept Mol & Cellular Neurobiol, CNCR, Amsterdam, Netherlands

[3] Vrije Univ Amsterdam, Dept Funct Genom, CNCR, Amsterdam, Netherlands

[4] Vrije Univ Amsterdam Med Ctr, Sect Complex Trait Genet, Dept Clin Genet, Amsterdam, Netherlands

[5] Vrije Univ Amsterdam Med Ctr, Dept Clin Genet, Amsterdam, Netherlands

来源：

PLOS ONE | 2014年 / 9卷 / 09期

关键词：

ETHANOL INTOXICATION; MICE; MODEL; ARCHITECTURE; BATTERY; DISEASE; ANXIETY;

D O I：

10.1371/journal.pone.0108563

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Functional genetic analyses in mice rely on efficient and in-depth characterization of the behavioral spectrum. Automated home-cage observation can provide a systematic and efficient screening method to detect unexplored, novel behavioral phenotypes. Here, we analyzed high-throughput automated home-cage data using existing and novel concepts, to detect a plethora of genetic differences in spontaneous behavior in a panel of commonly used inbred strains (129S1/SvImJ, A/J, C3H/HeJ, C57BL/6J, BALB/cJ, DBA/2J, NOD/LtJ, FVB/NJ, WSB/EiJ, PWK/PhJ and CAST/EiJ). Continuous video-tracking observations of sheltering behavior and locomotor activity were segmented into distinguishable behavioral elements, and studied at different time scales, yielding a set of 115 behavioral parameters of which 105 showed highly significant strain differences. This set of 115 parameters was highly dimensional; principal component analysis identified 26 orthogonal components with eigenvalues above one. Especially novel parameters of sheltering behavior and parameters describing aspects of motion of the mouse in the home-cage showed high genetic effect sizes. Multi-day habituation curves and patterns of behavior surrounding dark/light phase transitions showed striking strain differences, albeit with lower genetic effect sizes. This spontaneous home-cage behavior study demonstrates high dimensionality, with a strong genetic contribution to specific sets of behavioral measures. Importantly, spontaneous home-cage behavior analysis detects genetic effects that cannot be studied in conventional behavioral tests, showing that the inclusion of a few days of undisturbed, labor extensive home-cage assessment may greatly aid gene function analyses and drug target discovery.

引用

页数：9

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