A laboratory exercise in comparative DNA analysis

被引：2
作者：
Brown, LR [1 ]
机构：
[1] Goucher Coll, Dept Chem, Baltimore, MD 21204 USA
来源：
BIOCHEMISTRY AND MOLECULAR BIOLOGY EDUCATION | 2003年 / 31卷 / 03期
关键词：
plasmid purification; fluorometry; UV-visible spectroscopy;
D O I：
10.1002/bmb.2003.494031030210
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
This report describes a laboratory exercise that is used in an upper level biochemistry course at a small liberal arts college. The laboratory exercise was designed with three major learning objectives in mind. The first objective is that students learn how to isolate and purify plasmid DNA. The second is that they understand this process on a molecular level. Last, they analyze their DNA samples using a variety of biochemical and biophysical methods. By comparing and contrasting the results, they are to draw conclusions about which methods would be best under a given set of experimental conditions.
引用
收藏
页码：177 / 179
页数：3
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