Study of solid-phase time-resolved fluorescence label immunoassay

This paper describes optimal conditions for HBsAbIgG labeling with a new fluorescence probe, 4, 7-bis-chorosulfophenyl-1, 10-phenanthroline-2,9-dicarboxylic acid(BCPDA)for the solid phase time-resolved fluorimmunoassay (TRFIA). The result of experiment under states clearly that BCPDA may react with protein under relative mild condition. The relative bioactivity of reacted protein was more than 80%. The labeling molar ratio of BCPDA for HBsAbIgG was 45-70. The recovery was higher than 80%. Protein-BCPDA-Eu3+ complex is stable. It can emit very high fluorescence intensity with very long fluorescence life times. The fluorescence of Protein-BCPDA-Eu3+ complex has a very large stokes shift (270 nm). The emission band at 611.2 nm is very narrow. The research provides the base for developing non-isotopic immunoassay technique and clinical medical diagnosis.