The mechanism of the supercoiled DNA recognition by the eukaryotic type I topoisomerases: I. The enzyme interaction with nonspecific oligonucleotides

被引：11

作者：

Bugreev, DV

Buneva, VN

Sinitsyna, OI

Nevinsky, GA

机构：

[1] Russian Acad Sci, Novosibirsk Bioorgan Chem Inst, Siberian Div, Novosibirsk 630090, Russia

[2] Russian Acad Sci, Inst Cytol & Genet, Siberian Div, Novosibirsk 630090, Russia

来源：

RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY | 2003年 / 29卷 / 02期

基金：

俄罗斯基础研究基金会;

关键词：

DNA topoisomerase I from mice and human placenta; mechanism of interaction with nucleotides; and single- and double-stranded DNAs;

D O I：

10.1023/A:1023208431839

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Interaction of the DNA type I topoisomerases from the murine and human placenta cells with nonspecific oligonucleotides was analyzed. The contributions of strong and week nonspecific electrostatic, van der Waals's, and hydrophobic interactions, and hydrogen bonding of the enzymes to the complex formation with the single- and double-stranded DNAs were determined. The factors that determine the top-priority recognition of the topologically stressed DNA were revealed. The results were interpreted in comparison with the X-ray analysis data for human DNA topoisomerase I.

引用

页码：143 / 153

页数：11

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