Extracellular cAMP inhibits P2X3 receptors in rat sensory neurones through G protein-mediated mechanism

被引:4
作者
Mamenko, M. V. [1 ]
Chizhmakov, I. V. [1 ]
Volkova, T. M. [1 ]
Verkhratsky, A. [2 ,3 ]
Krishtal, O. A. [1 ]
机构
[1] Bogomoletz Inst Physiol, UA-01024 Kiev, Ukraine
[2] ASCR, Inst Expt Med, Prague, Czech Republic
[3] Univ Manchester, Fac Life Sci, Manchester, Lancs, England
关键词
ATP; cAMP; cAMP receptors; G proteins; purinergic transmission; sensory neurone; SOMATOSENSORY CORTEX; DICTYOSTELIUM; CHANNELS; SURFACE; SYSTEM; PHOSPHORYLATION; DESENSITIZATION; TRANSMISSION; MEMBRANE; BINDING;
D O I
10.1111/j.1748-1716.2010.02088.x
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Aim: To identify the mechanisms of P2X(3) receptor inhibition by extracellular cyclic adenosine monophosphate (cAMP) in rat dorsal root ganglion (DRG) neurones. Methods: Whole-cell currents were measured in cultured DRG neurones using the combination of voltage and concentration clamp. Results: We have found that extracellular cAMP inhibits P2X(3)-mediated currents in a concentration- and use-dependent manner. The P2X(3) currents, activated by ATP applied every 4 min, were inhibited by 55% in the presence of 10 mu m cAMP and by 81% in the presence of 30 mu m cAMP. At 8 min interval between ATP applications the same concentration of cAMP did not alter the currents. Addition of 0.5 mm of guanosine 5'-O-(2-thiodiphosphate) to intracellular solution blocked the inhibitory action of cAMP. The inhibitory effects of cAMP were not mimicked by extracellular application of 30 mu m adenosine. Conclusions: In this paper, we demonstrate, for the first time, that extracellular application of cAMP to rat sensory neurones inhibits P2X(3) receptors via a G protein-coupled mechanism in a use-dependent manner, thus indicating the neuronal expression of specific plasmalemmal cAMP receptor.
引用
收藏
页码:199 / 204
页数:6
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