Development of a High-Sensitivity Quantitation Method for Arginine Vasopressin by High-Performance Liquid Chromatography Tandem Mass Spectrometry, and Comparison with Quantitative Values by Radioimmunoassay

被引：4

作者：

Tsukazaki, Yasuko ^{[1
]}

Senda, Naoto ^{[1
,2
]}

Kubo, Kinya ^{[2
]}

Yamada, Shigeru ^{[3
]}

Kugoh, Hiroyuki ^{[2
,4
]}

Kazuki, Yasuhiro ^{[2
,4
]}

Oshimura, Mitsuo ^{[2
]}

机构：

[1] LSI Medience Corp, Analyt Technol Res Dept, 2-1-6 Sengen, Tsukuba, Ibaraki 3050047, Japan

[2] Tottori Univ, Chromosome Engn Res Ctr, 86 Nishi Cho, Yonago, Tottori 6838503, Japan

[3] KK AB SCIEX, 4-7-35 Kitashinagawa, Shinagawa, Tokyo 1400001, Japan

[4] Tottori Univ, Grad Sch Med Sci, Inst Regenerat Med & Biofunct, Dept Biomed Sci, 86 Nishi Cho, Yonago, Tottori 6838503, Japan

来源：

ANALYTICAL SCIENCES | 2016年 / 32卷 / 02期

关键词：

Arginine vasopressin; plasma; mass spectrometry; liquid chromatography; matrix effect; analytical sensitivity; analytical specificity; electrode; accuracy; radioimmunoassay; LC-MS/MS; ION SUPPRESSION; HUMAN PLASMA; OXYTOCIN; IONIZATION; QUANTIFICATION; IDENTIFICATION; ENHANCEMENT; URINE;

D O I：

10.2116/analsci.32.153

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Human plasma arginine vasopressin (AVP) levels serve as a clinically relevant marker of diabetes and related syndromes. We developed a highly sensitive method for measuring human plasma AVP using high-performance liquid chromatography tandem mass spectrometry. AVP was extracted from human plasma using a weak-cation solid-phase extraction plate, and separated on a wide-bore octadecyl reverse-phase column. AVP was quantified in ion-transition experiments utilizing a product ion (m/z 328.3) derived from its parent ion (m/z 542.8). The sensitivity was enhanced using 0.02% dichloromethane as a mobile-phase additive. The lower limit of quantitation was 0.200 pmol/L. The extraction recovery ranged from 70.2 +/- 7.2 to 73.3 +/- 6.2% (mean +/- SD), and the matrix effect ranged from 1.1 - 1.9%. Quality-testing samples revealed interday/intraday accuracy and precision ranging over 0.9 - 3% and 0.3 - 2%, respectively, which included the endogenous baseline. Our results correlated well with radioimmunoassay results using 22 human volunteer plasma samples.

引用

页码：153 / 159

页数：7