Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) strains isolated from dairy products: Relationship of ica-dependent/independent and components of biofilms produced in vitro

被引：10
作者：
Guadalupe Avila-Novoa, Maria [1 ]
Gonzalez-Gomez, Jean-Pierre [3 ]
Javier Guerrero-Medina, Pedro [2 ]
Antonio Cardona-Lopez, Marco [1 ]
Maria Ibarra-Velazquez, Luz [1 ]
Yolanda Velazquez-Suarez, Noemi [1 ]
Morales-del Rio, Juan-Alfredo [2 ]
Gutierrez-Lomeli, Melesio [2 ]
机构：
[1] Univ Guadalajara, Ctr Univ La Cienega, Div Desarrollo Biotecnol, Lab Microbiol, Av Univ 1115, Ocotlan, Jalisco, Mexico
[2] Univ Guadalajara, Ctr Univ La Cienega, Div Desarrollo Biotecnol, Ctr Invest Biotecnol Microbiana & Alimentaria, Av Univ 1115, Ocotlan, Jalisco, Mexico
[3] Ctr Invest Alimentac & Desarrollo AC CIAD, Lab Nacl Invest Inocuidad Alimentaria LANIIA, Carretera El Dorado Km 5-5, Culiacan, Sinaloa, Mexico
来源：
INTERNATIONAL DAIRY JOURNAL | 2021年 / 119卷
关键词：
FOOD CONTACT SURFACES; ANTIMICROBIAL SUSCEPTIBILITY; MOLECULAR CHARACTERIZATION; ANTIBIOTIC-RESISTANCE; ENTEROTOXIN GENES; STAINLESS-STEEL; BOVINE MASTITIS; MILK; COWS; PREVALENCE;
D O I：
10.1016/j.idairyj.2021.105066
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
This study investigated the prevalence of Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) in dairy products, and the relationship between polysaccharide intercellular adhesin (PIA) dependence/independence of MRSAs and the components of biofilms produced in vitro. Of 141 samples of dairy products collected, 26% were positive for S. aureus and 14% of S. aureus isolates were MRSA. MRSA biofilms (8.37-9.89 and 9.53-9.59 log(10) cfu cm(-2) for ica- positive and negative MRSA, respectively, in TSBNaCl on stainless steel) were characterised by epifluorescence microscopy, scanning electron microscopy, confocal laser scanning microscopy and biofilm detachment was assessed with DNase I (32-44%) and proteinase K (18-32%) (p < 0.05). MRSA biofilms isolated from dairy products are a source of food contamination, thus a need to implement food safety systems and strategies for biofilm prevention and removal in the dairy industry. (C) 2021 Elsevier Ltd. All rights reserved.
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页数：9
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