The Southeast Collaboratory for Structural Genomics: A high-throughput gene to structure factory

被引：60

作者：

Adams, MWW

Dailey, HA

Delucas, LJ

Luo, M

Prestegard, JH

Rose, JP

Wang, BC ^{[1
]}

机构：

[1] Univ Georgia, Dept Biochem & Mol Biol, Athens, GA 30602 USA

[2] Univ Alabama Birmingham, Dept Microbiol, Ctr Biophys Sci & Engn, Birmingham, AL 35294 USA

来源：

ACCOUNTS OF CHEMICAL RESEARCH | 2003年 / 36卷 / 03期

关键词：

D O I：

10.1021/ar0101382

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The Southeast Collaboratory for Structural Genomics consists of four working groups. The protein production group supplies/develops high-output production of Pyrococcus furiosus, Caenorhabditis elegans, and selected human proteins. The X-ray crystallography group conducts high-throughput structure production in parallel with production-related research/development in nanocrystallization robotics, capillary crystallization cassette, synchrotron/home X-ray instrumentation, sample mounting robotics, data processing and pipelined structure analysis, combined refinement/validation protocols, and direct use of unlabeled native crystals (Direct Crystallography). The NMR group emphasizes/develops sample screening and backbone structure determination from residual dipolar coupling data. The bioinformatics group implements/develops local database interfaces, pipelined sequence/structure information search/updates, and database/bioinformatics toolkits.

引用

收藏

页码：191 / 198

页数：8

相关论文

共 23 条

[1] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].

Altschul, SF ;

Madden, TL ;

Schaffer, AA ;

Zhang, JH ;

Zhang, Z ;

Miller, W ;

Lipman, DJ .

NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402

[2] The Protein Data Bank [J].

Berman, HM ;

Westbrook, J ;

Feng, Z ;

Gilliland, G ;

Bhat, TN ;

Weissig, H ;

Shindyalov, IN ;

Bourne, PE .

NUCLEIC ACIDS RESEARCH, 2000, 28 (01) :235-242

[3] Crystallography & NMR system:: A new software suite for macromolecular structure determination [J].

Brunger, AT ;

Adams, PD ;

Clore, GM ;

DeLano, WL ;

Gros, P ;

Grosse-Kunstleve, RW ;

Jiang, JS ;

Kuszewski, J ;

Nilges, M ;

Pannu, NS ;

Read, RJ ;

Rice, LM ;

Simonson, T ;

Warren, GL .

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1998, 54 :905-921

[4] Genome sequence of the nematode C-elegans:: A platform for investigating biology [J].

不详 .

SCIENCE, 1998, 282 (5396) :2012-2018

[5]

Fischer D, 2001, PROTEINS, P171

[6] Ab initio crystallographic structure determination of insulin from protein to electron density without crystal handling [J].

Gavira, JA ;

Toh, D ;

Lopéz-Jaramillo, J ;

García-Ruíz, JM ;

Ng, JD .

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2002, 58 :1147-1154

[7] Probability analysis of variational crystallization and its application to gp120, the exterior envelope glycoprotein of type 1 human immunodeficiency virus (HIV-1) [J].

Kwong, PD ;

Wyatt, R ;

Desjardins, E ;

Robinson, J ;

Culp, JS ;

Hellmig, BD ;

Sweet, RW ;

Sodroski, J ;

Hendrickson, WA .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (07) :4115-4123

[8] Current state of automated crystallographic data analysis [J].

Lamzin, VS ;

Perrakis, A .

NATURE STRUCTURAL BIOLOGY, 2000, 7 (Suppl 11) :978-981

[9] Crystal structure of the cytoskeleton-associated protein glycine-rich (CAP-Gly) domain [J].

Li, SL ;

Finley, J ;

Liu, ZJ ;

Qiu, SH ;

Chen, HL ;

Luan, CH ;

Carson, M ;

Tsao, J ;

Johnson, D ;

Lin, GD ;

Zhao, J ;

Thomas, W ;

Nagy, LA ;

Sha, BD ;

DeLucas, LJ ;

Wang, BC ;

Luo, M .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (50) :48596-48601

[10] Structure of the Ca2+-regulated photoprotein obelin at 1.7 Å resolution determined directly from its sulfur substructure [J].

Liu, ZJ ;

Vysotski, ES ;

Chen, CJ ;

Rose, JP ;

Lee, J ;

Wang, BC .

PROTEIN SCIENCE, 2000, 9 (11) :2085-2093