Raman microspectroscopy differentiates perinatal pathogens on ex vivo infected human fetal membrane tissues

被引:7
作者
Ayala, Oscar D. [1 ,2 ]
Doster, Ryan S. [3 ]
Manning, Shannon D. [4 ]
O'Brien, Christine M. [1 ,2 ]
Aronoff, David M. [3 ,5 ,6 ]
Gaddy, Jennifer A. [3 ,5 ,7 ]
Mahadevan-Jansen, Anita [1 ,2 ]
机构
[1] Vanderbilt Univ, Biophoton Ctr, 221 Kirkland Hall, Nashville, TN 37235 USA
[2] Vanderbilt Univ, Dept Biomed Engn, Nashville, TN 37235 USA
[3] Vanderbilt Univ, Med Ctr, Dept Med, Div Infect Dis, Nashville, TN USA
[4] Michigan State Univ, Dept Microbiol & Mol Genet, E Lansing, MI 48824 USA
[5] Vanderbilt Univ, Med Ctr, Dept Pathol Microbiol & Immunol, Nashville, TN USA
[6] Vanderbilt Univ, Med Ctr, Dept Obstet & Gynecol, Nashville, TN 37232 USA
[7] Tennessee Valley Healthcare Syst, Dept Vet Affairs, Nashville, TN USA
基金
美国国家卫生研究院;
关键词
biofilms; chorioamnionitis; GBS; Group B Streptococcus; Raman spectroscopy; Streptococcus agalactiae; GROUP-B STREPTOCOCCUS; DISEASE; IDENTIFICATION; SEQUENCE; PIGMENT; SEPSIS;
D O I
10.1002/jbio.201800449
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Streptococcus agalactiae, also known as Group B Streptococcus (GBS), is a major cause of chorioamnionitis and neonatal sepsis. This study evaluates Raman spectroscopy (RS) to identify spectral characteristics of infection and differentiate GBS from Escherichia coli and Staphylococcus aureus during ex vivo infection of human fetal membrane tissues. Unique spectral features were identified from colonies grown on agar and infected fetal membrane tissues. Multinomial logistic regression analysis accurately identified GBS infected tissues with 100.0% sensitivity and 88.9% specificity. Together, these findings support further investigation into the use of RS as an emerging microbiologic diagnostic tool and intrapartum screening test for GBS carriage.
引用
收藏
页数:7
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