Mass spectrometry imaging of rat brain sections: nanomolar sensitivity with MALDI versus nanometer resolution by TOF-SIMS

被引：98

作者：

Benabdellah, Farida ^{[1
]}

Seyer, Alexandre ^{[1
]}

Quinton, Loic ^{[2
]}

Touboul, David ^{[1
]}

Brunelle, Alain ^{[1
]}

Laprevote, Olivier ^{[3
]}

机构：

[1] CNRS, Inst Chim Subst Nat, UPR 2301, F-91198 Gif Sur Yvette, France

[2] Univ Liege, Ctr Anal Residus Traces GIGA R, Lab Spectrometrie Masse, B-4000 Liege, Belgium

[3] Univ Paris 05, Fac Pharm, IFR 71, F-75006 Paris, France

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2010年 / 396卷 / 01期

关键词：

Mass spectrometry imaging; MALDI; TOF-SIMS; Lipid; Rat brain; ASSISTED-LASER-DESORPTION/IONIZATION; ION MOBILITY-TOFMS; SECONDARY-ION; DESORPTION IONIZATION; STRUCTURAL-ANALYSIS; SAMPLE PREPARATION; MATRIX DEPOSITION; SURFACE-ANALYSIS; TISSUE; TIME;

D O I：

10.1007/s00216-009-3031-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Mass spectrometry imaging is becoming a more and more widely used method for chemical mapping of organic and inorganic compounds from various surfaces, especially tissue sections. Two main different techniques are now available: matrix-assisted laser desorption/ionizaton, where the sample, preliminary coated by an organic matrix, is analyzed by a UV laser beam; and secondary ion mass spectrometry, for which the target is directly submitted to a focused ion beam. Both techniques revealed excellent performances for lipid mapping of tissue surfaces. This article will discuss similarities, differences, and specificities of ion images generated by these two techniques in terms of sample preparation, sensitivity, ultimate spatial resolution, and structural analysis.

引用

页码：151 / 162

页数：12

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