Production and optimization of cellulase from Fusarium oxysporum by submerged fermentation

被引:0
作者
Ramanathan, G. [1 ]
Banupriya, S. [1 ]
Abirami, D. [1 ]
机构
[1] VHNSN Coll, Dept Microbiol, Virudunagar 626001, India
来源
JOURNAL OF SCIENTIFIC & INDUSTRIAL RESEARCH | 2010年 / 69卷 / 06期
关键词
Cellulose; CMC; Fermentation; Fusarium oxysporum; Glucosidase; PURIFICATION;
D O I
暂无
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
Fusarium oxysporum, isolated from infected tomato plant parts, produced maximum cellulase at optimum parameters (pH, 6.0; temp., 50 degrees C; and incubation period, 12 d) in cellulase enzyme production broth having 1% CMC (carboxy methyl cellulose) as a cellulose substrate. Activities of purified cellulases (mol wt, 24, 29 and 45 kDa) were stimulated by concentrations (0-70 mM) Na+ and Mg++, while EDTA inhibited enzyme activity at all concentrations.
引用
收藏
页码:454 / 459
页数:6
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