Single-Cell Transcriptomic Map of the Human and Mouse Bladders

被引：112

作者：

Yu, Zhenyuan ^{[1
,2
,4
,6
,7
]}

Liao, Jinling ^{[4
,6
,7
]}

Chen, Yang ^{[1
,2
,4
,6
,7
]}

Zou, Chunlin ^{[8
,9
]}

Zhang, Haiying ^{[4
,6
,7
]}

Cheng, Jiwen ^{[1
,2
,4
,6
,7
]}

Liu, Deyun ^{[1
,2
]}

Li, Tianyu ^{[1
,2
]}

Zhang, Qingyun ^{[1
,4
,6
,7
,10
]}

Li, Jiaping ^{[3
,11
,12
]}

Yang, Xiaobo ^{[4
,6
,7
]}

Ye, Yu ^{[1
,4
,6
,7
,13
]}

Huang, Zhiguang ^{[4
,6
,7
]}

Long, Xinyang ^{[4
,6
,7
]}

Yang, Rirong ^{[4
,5
,6
,7
]}

Mo, Zengnan ^{[1
,2
,4
,6
,7
]}

机构：

[1] Guangxi Med Univ, Affiliated Hosp 1, Inst Urol & Nephrol, Nanning, Peoples R China

[2] Guangxi Med Univ, Affiliated Hosp 1, Dept Urol, Nanning, Peoples R China

[3] Guangxi Med Univ, Affiliated Hosp 1, Dept Cardiol, Nanning, Peoples R China

[4] Guangxi Med Univ, Sch Preclin Med, Ctr Genom & Personalized Med, Nanning, Peoples R China

[5] Guangxi Med Univ, Sch Preclin Med, Dept Immunol, Nanning, Peoples R China

[6] Guangxi Collaborat Innovat Ctr Genom & Personaliz, Nanning, Peoples R China

[7] Guangxi Key Lab Coll & Univ, Guangxi Key Lab Genom & Personalized Med, Nanning, Peoples R China

[8] Minist Educ, Key Lab Longev & Ageing Related Dis, Nanning, Peoples R China

[9] Guangxi Med Univ, Ctr Translat Med, Nanning, Peoples R China

[10] Guangxi Med Univ, Affiliated Tumor Hosp, Dept Urol, Nanning, Peoples R China

[11] Guangxi Key Lab Precis Med Cardiocerebrovasc Dis, Nanning, Peoples R China

[12] Guangxi Clin Res Ctr Cardiocerebrovasc Dis, Nanning, Peoples R China

[13] Guangxi Med Univ, Affiliated Hosp 2, Sci Res Dept, Nanning, Peoples R China

来源：

JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY | 2019年 / 30卷 / 11期

基金：

中国国家自然科学基金; 国家重点研发计划;

关键词：

single-cell RNA sequencing; single-cell transcriptomic map; bladder cells; interstitial cells; epithelial cells; INTERSTITIAL-CELLS; URINARY-BLADDER; EXPRESSION; TISSUE; BETA; BETA(3)-ADRENOCEPTORS; DIFFERENTIATION; REGULATORS; INFECTION; PROTEIN;

D O I：

10.1681/ASN.2019040335

中图分类号：

R5 [内科学]; R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

1002 ; 100201 ;

摘要：

Background Having a comprehensive map of the cellular anatomy of the normal human bladder is vital to understanding the cellular origins of benign bladder disease and bladder cancer. Methods We used single-cell RNA sequencing (scRNA-seq) of 12,423 cells from healthy human bladder tissue samples taken from patients with bladder cancer and 12,884 cells from mouse bladders to classify bladder cell types and their underlying functions. Results We created a single-cell transcriptomic map of human and mouse bladders, including 16 clusters of human bladder cells and 15 clusters of mouse bladder cells. The homology and heterogeneity of human and mouse bladder cell types were compared and both conservative and heterogeneous aspects of human and mouse bladder evolution were identified. We also discovered two novel types of human bladder cells. One type is ADRA2A(+) and HRH2(+) interstitial cells which may be associated with nerve conduction and allergic reactions. The other type is TNNT1(+) epithelial cells that may be involved with bladder emptying. We verify these TNNT1(+) epithelial cells also occur in rat and mouse bladders. Conclusions This transcriptomic map provides a resource for studying bladder cell types, specific cell markers, signaling receptors, and genes that will help us to learn more about the relationship between bladder cell types and diseases.

引用

页码：2159 / 2176

页数：18

共 56 条

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