Hyperglycemia increases the levels of vascular cellular adhesion molecule-1 and monocyte-chemoattractant-protein-1 in the diabetic endothelial cell

被引：60

作者：

Haubner, Frank ^{[1
]}

Lehle, Karla ^{[1
]}

Muenzel, Daniela ^{[1
]}

Schmid, Christof ^{[1
]}

Birnbaum, Dietrich E. ^{[1
]}

Preuner, Juergen G. ^{[1
]}

机构：

[1] Univ Hosp Regensburg, Dept Cardiothorac Surg, D-93042 Regensburg, Germany

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2007年 / 360卷 / 03期

关键词：

endothelial cell; diabetes mellitus; glucose; cell growth; adhesion molecules; cytokines;

D O I：

10.1016/j.bbrc.2007.06.044

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hyperglycemia is the major cause of diabetic angiopathy. Aim of our study was to evaluate the impact of high glucose on cell growth and function of human "diabetic" endothelial cells (EC). Incubation of non-diabetic EC with glucose moderately inhibited cell growth and increased the expression of ICAM-1 and E-selectin. In the disease-specific EC, glucose treatment resulted also in moderately inhibited cell growth by 5-10%, increased basal expression of VCAM-1 by 10-20%, and an enhanced release of monocyte-chemoattractant-protein-1 (MCP-1) by 40-70%. The expression of ICAM-1 and E-selectin and the release of IL-6 and IL-8 was not affected. The usage of our disease-specific EC model might evaluate the impact of systemic factors of diabetic patients in the progression of endothelial dysfunction, and may be suitable to develop relevant therapeutic regimens. (c) 2007 Elsevier Inc. All rights reserved.

引用

页码：560 / 565

页数：6

共 33 条

[1] ADHESION MOLECULES AND INFLAMMATORY INJURY [J].

ALBELDA, SM ;

SMITH, CW ;

WARD, PA .

FASEB JOURNAL, 1994, 8 (08) :504-512

[2] Effect of high glucose concentrations on expression of ELAM-1, VCAM-1 and ICAM-1 in HUVEC with and without cytokine activation [J].

Altannavch, TS ;

Roubalová, K ;

Kucera, P ;

Andel, M .

PHYSIOLOGICAL RESEARCH, 2004, 53 (01) :77-82

[3]

Altannavch TS, 2002, ACTA VIROL, V46, P183

[4] Relation between glycaemic control, hyperinsulinaemia and plasma concentrations of soluble adhesion molecules in patients with impaired glucose tolerance or Type II diabetes [J].

Blüher, M ;

Unger, R ;

Rassoul, F ;

Richter, V ;

Paschke, R .

DIABETOLOGIA, 2002, 45 (02) :210-216

[5] Circulating adhesion molecules levels in type 2 diabetes mellitus and hypertension [J].

Boulbou, MS ;

Koukoulis, GN ;

Makri, ED ;

Petinaki, EA ;

Gourgoulianis, KI ;

Germenis, AE .

INTERNATIONAL JOURNAL OF CARDIOLOGY, 2005, 98 (01) :39-44

[6] The possible role of postprandial hyperglycaemia in the pathogenesis of diabetic complications [J].

Ceriello, A .

DIABETOLOGIA, 2003, 46 (Suppl 1) :M9-M16

[7] Adhesion of monocytes to arterial endothelium and initiation of atherosclerosis are critically dependent on vascular cell adhesion molecule-1 gene dosage [J].

Dansky, HM ;

Barlow, CB ;

Lominska, C ;

Sikes, JL ;

Kao, C ;

Weinsaft, J ;

Cybulsky, MI ;

Smith, JD .

ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2001, 21 (10) :1662-1667

[8] Long-term exposure to high glucose up-regulates VCAM-induced endothelial cell adhesiveness to PBMC [J].

Esposito, C ;

Fasoli, G ;

Plati, A ;

Bellotti, N ;

Conte, MM ;

Cornacchia, F ;

Foschi, A ;

Mazzullo, T ;

Semeraro, L ;

Dal Canton, A .

KIDNEY INTERNATIONAL, 2001, 59 (05) :1842-1849

[9] Elevated concentrations of circulating adhesion molecules and their association with microvascular complications in insulin-dependent diabetes mellitus [J].

Fasching, P ;

Veitl, M ;

Rohac, M ;

Streli, C ;

Schneider, B ;

Waldhausl, W ;

Wagner, OF .

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1996, 81 (12) :4313-4317

[10] Fas and Fas ligand in embryos and adult mice: Ligand expression in several immune-privileged tissues and coexpression in adult tissues characterized by apoptotic cell turnover [J].

French, LE ;

Hahne, M ;

Viard, I ;

Radlgruber, G ;

Zanone, R ;

Becker, K ;

Muller, C ;

Tschopp, J .

JOURNAL OF CELL BIOLOGY, 1996, 133 (02) :335-343

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