Norepinephrine-induced calcium signaling in astrocytes in the respiratory network of the ventrolateral medulla

被引：11

作者：

Schnell, Christian ^{[1
,3
,4
]}

Negm, Mahmoud ^{[2
]}

Driehaus, Johannes ^{[2
]}

Scheller, Anja ^{[5
]}

Huelsmann, Swen ^{[1
,2
]}

机构：

[1] DFG Ctr Nanoscale Microscopy & Mol Physiol Brain, Gottingen, Germany

[2] Univ Hosp Gottingen, Anesthesiol Clin, D-37099 Gottingen, Germany

[3] Cardiff Univ, Sch Biosci, Div Pathophysiol, Cardiff CF10 3AX, S Glam, Wales

[4] Cardiff Univ, Sch Biosci, Div Repair & Neurosci, Cardiff CF10 3AX, S Glam, Wales

[5] Univ Saarland, CIPMM, Mol Physiol, Homburg, Germany

来源：

RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY | 2016年 / 226卷

关键词：

Glia; Catecholaminergic signaling; Neuronal control of breathing; NORADRENERGIC MODULATION; RHYTHM GENERATOR; TAMOXIFEN; RAT; EXPRESSION; GLIA; RESPONSES; INFLUX;

D O I：

10.1016/j.resp.2015.10.008

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

The neuronal activity in the respiratory network of the ventrolateral medulla strongly depends on a variety of different neuromodulators. Since the respiratory activity generated by neurons in the pre-Botzinger complex (preBotC) is stabilized by astrocytes, we investigated potential effects of the neuromodulator norepinephrine (NE) on the astrocytic calcium signaling in the ventral respiratory group. In acutely isolated brainstem slices from wild type mice (postnatal day 1-10) we performed calcium imaging experiments using Oregon Green 488 BAPTA-1 AM as a calcium indicator dye. Astrocytes in the preBotC, which were identified by their unique intracellular calcium rise after the reduction of the extracellular K+ concentration, showed calcium rises in response to norepinephrine. These calcium signals persisted after blockade of neuronal activity by tetrodotoxin (TTX) indicating that they were independent of neuronal activity. Furthermore, application of the endoplasmic reticulum calcium pump blocker cyclopiazonic acid (CPA) diminished norepinephrine-induced calcium signals. This results could be confirmed using trans genic mice with astrocyte specific expression of GCaMP3. Thus, norepinephrine might, apart from acting directly on neurons, influence and modulate respiratory network activity via the modulation of astroglial calcium signaling. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：18 / 23

页数：6

共 41 条

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