Follicular lymphoma can be distinguished from benign follicular hyperplasia by flow cytometry using simultaneous staining of cytoplasmic bcl-2 and cell surface CD20

被引:25
作者
Cornfield, DB
Mitchell, DM
Almasri, NM
Anderson, JB
Ahrens, KP
Dooley, EO
Braylan, RC
机构
[1] Univ Florida, Coll Med, Dept Pathol & Lab Med, Hematopathol Sect, Gainesville, FL 32610 USA
[2] Univ Florida, Coll Med, Shands Hosp, Gainesville, FL 32610 USA
关键词
flow cytometry; bcl-2; follicular lymphoma; follicular hyperplasia;
D O I
10.1309/VJTK-DJWA-X297-XF8Q
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
The distinction between benign follicular hyperplasia (FH) and follicular lymphoma (FL) is sometimes problematic. We wanted to determine whether the expression of bcl-2 of FH was quantitatively different from that of FL, using surface CD20 expression as a discriminator of the various lymphoid compartments. Lymph node cell suspensions from 12 cases of FH and 17 cases of FL were analyzed by flow cytometry using a combined surface CD20 and intracellular bcl-2 staining. CD20- T cells in FH demonstrated the same bcl-2 expression as the CD20+ mantle cells, but the bright CD20+ germinal center cells showed near absence of bcl-2 expression. In contrast, the neoplastic cells of FL showed greater bcl-2 expression than the T cells of the same tumors and all cell populations of FH. This difference was particularly significant between the neoplastic B cells of FL and the germinal center cells of FH. The combined analysis of CD20 and bcl-2 should be useful for the differential diagnosis between FH and FL and particularly applicable to limited samples or when B-cell clonality is in question. Whether the quantitation of bcl-2 expression can be of further discriminatory value in malignant lymphomas remains to be determined.
引用
收藏
页码:258 / 263
页数:6
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