A simple dual-response fluorescent probe for imaging of viscosity and ONOO- through different fluorescence signals in living cells and zebrafish

被引：30

作者：

Wang, Chong ^{[1
]}

Shu, Wei ^{[1
]}

Chen, Qianqian ^{[1
]}

Yang, Chunlei ^{[1
]}

Su, Sa ^{[1
]}

Gao, Mengxu ^{[1
]}

Zhang, Rubo ^{[1
]}

Jing, Jing ^{[1
]}

Zhang, Xiaoling ^{[1
]}

机构：

[1] Beijing Inst Technol, Beijing Key Lab Photoelect Electrophoton Convers, Key Lab Med Mol Sci & Pharmaceut Engn,Minist Ind, Key Lab Cluster Sci,Minist Educ,Analyt & Testing, Beijing 100081, Peoples R China

来源：

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2021年 / 260卷

基金：

中国国家自然科学基金;

关键词：

Fluorescent probe; Peroxynitrite; Viscosity; Bioimaging; PEROXYNITRITE; MITOCHONDRIA; VISUALIZATION; BIOCHEMISTRY; DAMAGE; MODEL;

D O I：

10.1016/j.saa.2021.119990

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

Cellular viscosity is a prominent micro-environmental parameter and peroxynitrite is an essential reactive oxygen special, both of which are involved in various pathological and physiological processes. When the intracellular viscosity is abnormal or the ONOO- concentration is irregular, the normal function of cells will be disturbed. Herein, we rationally designed and synthesized a novel multichannel fluorescent probe (probe 1) for multichannel imaging of viscosity and peroxynitrite. Probe 1 displayed about 108-fold enhancement as the viscosity increased from 1.005 cP to 1090 cP. Moreover, the fluorescence inten-sity at 540 nm was quickly increased after adding ONOO-. It should be noted that probe 1 has high sen-sitivity, selectivity and low cytotoxicity, which can be successfully employed for the visualization of exogenous and endogenous ONOO- and imaging viscosity changes in HeLa cells by different fluorescent signals. Furthermore, probe 1 could monitor the change of ONOO- induced by LPS (lipopolysaccharide) and IFN-gamma (interferon-gamma) in zebrafish. This result reveals that probe 1 may inspire more diagnostic and therapeutic programs for viscosity-peroxynitrite related diseases shortly. (C) 2021 Elsevier B.V. All rights reserved.

引用

页数：8

共 45 条

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