Phenotypic and Genotypic Characteristics of a Tigecycline-Resistant Acinetobacter pittii Isolate Carrying blaNDM-1 and the Novel blaOXA Allelic Variant blaOXA-1045

被引:5
作者
Ding, Zixuan [1 ]
Li, Zhaoyinqian [1 ]
Zhao, Yuanqing [1 ]
Hao, Jingchen [1 ]
Li, Tingting [1 ]
Liu, Yao [1 ]
Zeng, Zhangrui [1 ]
Liu, Jinbo [1 ]
机构
[1] Affiliated Hosp Southwest Med Univ, Dept Lab Med, Luzhou, Peoples R China
关键词
Acinetobacter pittii; tigecycline resistance; carbapenem resistance; OXA-1045; whole genome sequencing; BETA-LACTAMASE GENES; INTENSIVE-CARE-UNIT; MOLECULAR EPIDEMIOLOGY; CARBAPENEM RESISTANCE; BIOFILM FORMATION; BAUMANNII; PATIENT; SPP; SUSCEPTIBILITY; IDENTIFICATION;
D O I
10.3389/fmicb.2022.868152
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A tigecycline-resistant Acinetobacter pittii clinical strain from pleural fluid carrying a bla(NDM-1) gene and a novel bla(OXA) gene, bla(OXA-1045), was isolated and characterized. The AP2044 strain acquired two copies of the bla(NDM-1) gene and six antibiotic resistance genes (ARGs) from other pathogens. According to the whole-genome investigation, the GC ratios of ARGs (50-60%) were greater than those of the chromosomal backbone (39.46%), indicating that ARGs were horizontally transferred. OXA-1045 belonged to the OXA-213 subfamily and the amino acid sequence of OXA-1045 showed 89% similarity to the amino acid sequences of OXA-213. Then, bla(OXA-1045) and bla(OXA-213) were cloned and the minimum inhibitory concentrations (MICs) of beta-lactams in the transformants were determined using the broth microdilution method. OXA-1045 was able to confer a reduced susceptibility to piperacillin and piperacillin-tazobactam compared to OXA-213. AP2044 strain exhibited low pathogenicity in Galleria mellonella infection models. The observation of condensed biofilm using the crystal violet staining method and scanning electron microscopy (SEM) suggested that the AP2044 strain was a weak biofilm producer. Quantitative reverse transcription-PCR (qRT-PCR) was used to detect the expression of resistance-nodulation-cell division (RND) efflux pump-related genes. The transcription level of adeB and adeJ genes increased significantly and was correlated with tigecycline resistance. Therefore, our genomic and phenotypic investigations revealed that the AP2044 strain had significant genome plasticity and natural transformation potential, and the emergence of antibiotic resistance in these unusual bacteria should be a concern for future investigations.
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页数:12
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共 69 条
[1]   Comparative Genome Sequence Analysis of Multidrug-Resistant Acinetobacter baumannii [J].
Adams, Mark D. ;
Goglin, Karrie ;
Molyneaux, Neil ;
Hujer, Kristine M. ;
Lavender, Heather ;
Jamison, Jennifer J. ;
MacDonald, Ian J. ;
Martin, Kristienna M. ;
Russo, Thomas ;
Campagnari, Anthony A. ;
Hujer, Andrea M. ;
Bonomo, Robert A. ;
Gill, Steven R. .
JOURNAL OF BACTERIOLOGY, 2008, 190 (24) :8053-8064
[2]   Molecular Epidemiology of Carbapenem-Nonsusceptible Acinetobacter baumannii in the United States [J].
Adams-Haduch, Jennifer M. ;
Onuoha, Ezenwa O. ;
Bogdanovich, Tatiana ;
Tian, Guo-Bao ;
Marschall, Jonas ;
Urban, Carl M. ;
Spellberg, Brad J. ;
Rhee, Diane ;
Halstead, Diane C. ;
Pasculle, Anthony W. ;
Doi, Yohei .
JOURNAL OF CLINICAL MICROBIOLOGY, 2011, 49 (11) :3849-3854
[3]   Reservoirs of Non-baumannii Acinetobacter Species [J].
Al Atrouni, Ahmad ;
Joly-Guillou, Marie-Laure ;
Hamze, Monzer ;
Kempf, Marie .
FRONTIERS IN MICROBIOLOGY, 2016, 7
[4]   BLAST Ring Image Generator (BRIG): simple prokaryote genome comparisons [J].
Alikhan, Nabil-Fareed ;
Petty, Nicola K. ;
Ben Zakour, Nouri L. ;
Beatson, Scott A. .
BMC GENOMICS, 2011, 12
[5]   Acinetobacter spp. as nosocomial pathogens: Epidemiology and resistance features [J].
Almasaudi, Saad B. .
SAUDI JOURNAL OF BIOLOGICAL SCIENCES, 2018, 25 (03) :586-596
[6]   IncM Plasmid R1215 Is the Source of Chromosomally Located Regions Containing Multiple Antibiotic Resistance Genes in the Globally Disseminated Acinetobacter baumannii GC1 and GC2 Clones [J].
Blackwell, Grace A. ;
Hamidian, Mohammad ;
Hall, Ruth M. .
MSPHERE, 2016, 1 (03)
[7]   Dissemination of New Delhi metallo-ß-lactamase-1-producing Acinetobacter baumannii in Europe [J].
Bonnin, R. A. ;
Poirel, L. ;
Naas, T. ;
Pirs, M. ;
Seme, K. ;
Schrenzel, J. ;
Nordmann, P. .
CLINICAL MICROBIOLOGY AND INFECTION, 2012, 18 (09) :E362-E365
[8]   New Delhi metallo-β-lactamase-producing Acinetobacter baumannii: a novel paradigm for spreading antibiotic resistance genes [J].
Bonnin, Remy A. ;
Poirel, Laurent ;
Nordmann, Patrice .
FUTURE MICROBIOLOGY, 2014, 9 (01) :33-41
[9]   First Identification of OXA-72 Carbapenemase from Acinetobacter pittii in Colombia [J].
Camila Montealegre, Maria ;
Jose Maya, Juan ;
Correa, Adriana ;
Espinal, Paula ;
Mojica, Maria F. ;
Ruiz, Sory J. ;
Rosso, Fernando ;
Vila, Jordi ;
Quinn, John P. ;
Virginia Villegas, Maria .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2012, 56 (07) :3996-3998
[10]   AdeIJK, a resistance-nodulation-cell division pump effluxing multiple antibiotics in Acinetobacter baumannii [J].
Damier-Piolle, Laurence ;
Magnet, Sophie ;
Bremont, Sylvie ;
Lambert, Thierry ;
Courvalin, Patrice .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2008, 52 (02) :557-562