Investigation on interaction of prulifloxacin with pepsin: A spectroscopic analysis

被引：42

作者：

Huang, Yabei ^{[1
,2
]}

Yan, Jie ^{[2
]}

Liu, Benzhi ^{[1
]}

Yu, Zhang ^{[1
]}

Gao, Xiaoyan ^{[1
]}

Tang, Yingcai ^{[1
]}

Zi, Yanqin ^{[1
]}

机构：

[1] Huaibei Coal Normal Coll, Dept Chem, Huaibei 235000, Anhui, Peoples R China

[2] Huaibei Occupat Dis Prevent & Cure Hosp, Huaibei 235000, Anhui, Peoples R China

来源：

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2010年 / 75卷 / 03期

关键词：

Prulifloxacin; Pepsin; Fluorescence quenching; Stern-Volmer equation; The Forster's resonance energy transfer theory; BOVINE SERUM-ALBUMIN; FLUORESCENCE SPECTROSCOPY; GASTRIC-CANCER; ACTIVE-SITE; FLUOROQUINOLONE; CIPROFLOXACIN; QUINOLONE; INVITRO; BINDING; OXYGEN;

D O I：

10.1016/j.saa.2009.12.044

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

The interaction between prulifloxacin, a kind of new oral taking antibiotic and pepsin, a kind of enzyme in the stomach has been investigated in vitro under a simulated physiological condition by different spectroscopic methods. The intrinsic fluorescence of pepsin was strongly quenched by prulifloxacin. This effect was rationalized in terms of a static quenching procedure. The binding parameters have been evaluated by fluorescence quenching methods. The negative value of AGO reveals that the binding process is a spontaneous process. The binding distance R between donor (pepsin) and acceptor (prulifloxacin) was obtained according to the Forster's resonance energy transfer theory and found to be 0.95 nm. The results obtained herein will be of biological significance in pharmacology and clinical medicine. Crown Copyright (C) 2010 Published by Elsevier B.V. All rights reserved.

引用

页码：1024 / 1029

页数：6

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