The growth, survival and thermal inactivation of Escherichia coli O157:H7 in a traditional South African sausage

This study investigated the growth and survival of Escherichia coli O157:H7 inoculated into boerewors models with (B + P) and without (B - P) sulphur dioxide preservative at a low (L) and high (H) inoculum followed by storage at 0, 4 and 10 degrees C for 10 days. The pathogen's thermal inactivation at 50, 60, 65 and 70 degrees C was also evaluated in B + P. The B P at both low and high inocula had significantly higher recoveries at all temperatures compared to B + P. The BL + P and BH + P had significant reductions in recoveries at 0 degrees C, declining to below detectable limits at days 8 and 10, respectively. At 4 degrees C, the BL + P and BH + P recoveries declined significantly at day 10. At 10 degrees C, significant increases were observed from days 0 to day 10 in both models and at low and high inocula. At 0 degrees C, the BL - P and BH - P treatments had significant declines in recoveries. The combination of sulphur dioxide preservative and low temperature demonstrated the best efficacy against E. colt O157:H7 survival. Thermal inactivation of E. coli O157:H7 was 60 min at 60 degrees C, 80 s at 65 degrees C and 60 s at 70 degrees C. This study demonstrated that E. coli O157:H7 can survive in boerewors with and without preservative and is more sensitive to heat treatment at 70 degrees C. (C) 2009 Elsevier Ltd. All rights reserved.