The nucleoprotein is required for efficient coronavirus genome replication

被引：170
作者：
Almazán, F [1 ]
Galán, C [1 ]
Enjuanes, L [1 ]
机构：
[1] CSIC, Dept Mol & Cell Biol, Ctr Nacl Biotecnol, E-28049 Madrid, Spain
来源：
JOURNAL OF VIROLOGY | 2004年 / 78卷 / 22期
关键词：
D O I：
10.1128/JVI.78.22.12683-12688.2004
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The construction of a set of transmissible gastroenteritis coronavirus (TGEV)-derived replicons as bacterial artificial chromosomes is reported. These replicons were generated by sequential deletion of nonessential genes for virus replication, using a modified TGEV full-length cDNA clone containing unique restriction sites between each pair of consecutive genes. Efficient activity of TGEV replicons was associated with the presence of the nucleoprotein provided either in cis or in trans. TGEV replicons were functional in several cell lines, including the human cell line 293T, in which no or very low cytopathic effect was observed, and expressed high amounts of heterologous protein.
引用
收藏
页码：12683 / 12688
页数：6
相关论文
共 39 条
[11]   Poliovirus RNA replication requires genome circularization through a protein-protein bridge [J].
Herold, J ;
Andino, R .
MOLECULAR CELL, 2001, 7 (03) :581-591
[12]   The SARS coronavirus: A postgenomic era [J].
Holmes, KV ;
Enjuanes, L .
SCIENCE, 2003, 300 (5624) :1377-1378
[13]   Replication and packaging of transmissible gastroenteritis coronavirus-derived synthetic minigenomes [J].
Izeta, A ;
Smerdou, C ;
Alonso, S ;
Penzes, Z ;
Mendez, A ;
Plana-Durán, J ;
Enjuanes, L .
JOURNAL OF VIROLOGY, 1999, 73 (02) :1535-1545
[14]   Subgenomic replicons of the flavivirus Kunjin: Construction and applications [J].
Khromykh, AA ;
Westaway, EG .
JOURNAL OF VIROLOGY, 1997, 71 (02) :1497-1505
[15]   A novel coronavirus associated with severe acute respiratory syndrome [J].
Ksiazek, TG ;
Erdman, D ;
Goldsmith, CS ;
Zaki, SR ;
Peret, T ;
Emery, S ;
Tong, SX ;
Urbani, C ;
Comer, JA ;
Lim, W ;
Rollin, PE ;
Dowell, SF ;
Ling, AE ;
Humphrey, CD ;
Shieh, WJ ;
Guarner, J ;
Paddock, CD ;
Rota, P ;
Fields, B ;
DeRisi, J ;
Yang, JY ;
Cox, N ;
Hughes, JM ;
LeDuc, JW ;
Bellini, WJ ;
Anderson, LJ .
NEW ENGLAND JOURNAL OF MEDICINE, 2003, 348 (20) :1953-1966
[16]   Newly discovered coronavirus as the primary cause of severe acute respiratory syndrome [J].
Kuiken, T ;
Fouchier, RAM ;
Schutten, M ;
Rimmelzwaan, GF ;
van Amerongen, G ;
van Riel, D ;
Laman, JD ;
de Jong, T ;
van Doornum, G ;
Lim, W ;
Ling, AE ;
Chan, PKS ;
Tam, JS ;
Zambon, MC ;
Gopal, R ;
Drosten, C ;
van der Werf, S ;
Escriou, N ;
Manuguerra, JC ;
Stöhr, K ;
Peiris, JSM ;
Osterhaus, ADME .
LANCET, 2003, 362 (9380) :263-270
[17]   Replication of subgenomic hepatitis C virus RNAs in a hepatoma cell line [J].
Lohmann, V ;
Körner, F ;
Koch, JO ;
Herian, U ;
Theilmann, L ;
Bartenschlager, R .
SCIENCE, 1999, 285 (5424) :110-113
[18]   The genome sequence of the SARS-associated coronavirus [J].
Marra, MA ;
Jones, SJM ;
Astell, CR ;
Holt, RA ;
Brooks-Wilson, A ;
Butterfield, YSN ;
Khattra, J ;
Asano, JK ;
Barber, SA ;
Chan, SY ;
Cloutier, A ;
Coughlin, SM ;
Freeman, D ;
Girn, N ;
Griffith, OL ;
Leach, SR ;
Mayo, M ;
McDonald, H ;
Montgomery, SB ;
Pandoh, PK ;
Petrescu, AS ;
Robertson, AG ;
Schein, JE ;
Siddiqui, A ;
Smailus, DE ;
Stott, JE ;
Yang, GS ;
Plummer, F ;
Andonov, A ;
Artsob, H ;
Bastien, N ;
Bernard, K ;
Booth, TF ;
Bowness, D ;
Czub, M ;
Drebot, M ;
Fernando, L ;
Flick, R ;
Garbutt, M ;
Gray, M ;
Grolla, A ;
Jones, S ;
Feldmann, H ;
Meyers, A ;
Kabani, A ;
Li, Y ;
Normand, S ;
Stroher, U ;
Tipples, GA ;
Tyler, S .
SCIENCE, 2003, 300 (5624) :1399-1404
[19]   Reverse genetics of the largest RNA viruses [J].
Masters, PS .
ADVANCES IN VIRUS RESEARCH, VOL 53, 1999, 53 :245-264
[20]   The arterivirus replicase is the only viral protein required for genome replication and subgenomic mRNA transcription [J].
Molenkamp, R ;
van Tol, H ;
Rozier, BCD ;
van der Meer, Y ;
Spaan, WJM ;
Snijder, EJ .
JOURNAL OF GENERAL VIROLOGY, 2000, 81 :2491-2496
1234