The nucleoprotein is required for efficient coronavirus genome replication

被引：170

作者：

Almazán, F ^{[1
]}

Galán, C ^{[1
]}

Enjuanes, L ^{[1
]}

机构：

[1] CSIC, Dept Mol & Cell Biol, Ctr Nacl Biotecnol, E-28049 Madrid, Spain

来源：

JOURNAL OF VIROLOGY | 2004年 / 78卷 / 22期

关键词：

D O I：

10.1128/JVI.78.22.12683-12688.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The construction of a set of transmissible gastroenteritis coronavirus (TGEV)-derived replicons as bacterial artificial chromosomes is reported. These replicons were generated by sequential deletion of nonessential genes for virus replication, using a modified TGEV full-length cDNA clone containing unique restriction sites between each pair of consecutive genes. Efficient activity of TGEV replicons was associated with the presence of the nucleoprotein provided either in cis or in trans. TGEV replicons were functional in several cell lines, including the human cell line 293T, in which no or very low cytopathic effect was observed, and expressed high amounts of heterologous protein.

引用

页码：12683 / 12688

页数：6

共 39 条

[1] Noncytopathic Sindbis virus RNA vectors for heterologous gene expression [J].

Agapov, EV ;

Frolov, I ;

Lindenbach, BD ;

Pragai, BM ;

Schlesinger, S ;

Rice, CM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (22) :12989-12994

[2] Engineering the largest RNA virus genome as an infectious bacterial artificial chromosome [J].

Almazán, F ;

González, JM ;

Pénzes, Z ;

Izeta, A ;

Calvo, E ;

Plana-Durán, J ;

Enjuanes, L .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (10) :5516-5521

[3] Transcription regulatory sequences and mRNA expression levels in the coronavirus transmissible gastroenteritis virus [J].

Alonso, S ;

Izeta, A ;

Sola, I ;

Enjuanes, L .

JOURNAL OF VIROLOGY, 2002, 76 (03) :1293-1308

[4] Reverse genetics system for the avian coronavirus infectious bronchitis virus [J].

Casais, R ;

Thiel, V ;

Siddell, SG ;

Cavanagh, D ;

Britton, P .

JOURNAL OF VIROLOGY, 2001, 75 (24) :12359-12369

[5]

DELMAS B, 1993, ADV EXP MED BIOL, V342, P293

[6] Identification of a novel coronavirus in patients with severe acute respiratory syndrome [J].

Drosten, C ;

Günther, S ;

Preiser, W ;

van der Werf, S ;

Brodt, HR ;

Becker, S ;

Rabenau, H ;

Panning, M ;

Kolesnikova, L ;

Fouchier, RAM ;

Berger, A ;

Burguière, AM ;

Cinatl, J ;

Eickmann, M ;

Escriou, N ;

Grywna, K ;

Kramme, S ;

Manuguerra, JC ;

Müller, S ;

Rickerts, V ;

Stürmer, M ;

Vieth, S ;

Klenk, HD ;

Osterhaus, ADME ;

Schmitz, H ;

Doerr, HW .

NEW ENGLAND JOURNAL OF MEDICINE, 2003, 348 (20) :1967-1976

[7]

Enjuanes L., 2000, VIRUS TAXONOMY CLASS, P827

[8] Aetiology -: Koch's postulates fulfilled for SARS virus [J].

Fouchier, RAM ;

Kuiken, T ;

Schutten, M ;

van Amerongen, G ;

van Doornum, J ;

van den Hoogen, BG ;

Peiris, M ;

Lim, W ;

Stöhr, K ;

Osterhaus, ADME .

NATURE, 2003, 423 (6937) :240-240

[9] Alphavirus-based expression vectors: Strategies and applications [J].

Frolov, I ;

Hoffman, TA ;

Pragai, BM ;

Dryga, SA ;

Huang, HV ;

Schlesinger, S ;

Rice, CM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (21) :11371-11377

[10] Stabilization of a full-length infectious cDNA clone of transmissible gastroenteritis coronavirus by insertion of an intron [J].

González, JM ;

Pénzes, Z ;

Almazán, F ;

Calvo, E ;

Enjuanes, L .

JOURNAL OF VIROLOGY, 2002, 76 (09) :4655-4661

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