Molecular detection of trypanosomatids in neotropical primates in the state of Mato Grosso, Midwest, Brazil

被引：0

作者：

Candido, Stefhano Luis ^{[1
]}

Dandolini Pavelegini, Lucas Avelino ^{[2
]}

Pacheco, Thabata dos Anjos ^{[3
]}

Pacheco, Richard de Campos ^{[3
]}

de Barros Silva, Victoria Luiza ^{[3
]}

Morgado, Thais Oliveira ^{[4
]}

Colodel, Edson Moleta ^{[2
]}

Nakazato, Luciano ^{[1
]}

Parto Ferreira de Almeida, Arleana do Bom ^{[5
]}

Dutra, Valeria ^{[1
]}

机构：

[1] Univ Fed Mato Grosso UFMT, Hosp Vet HOVET, Fac Med Vet FAVET, Lab Microbiol & Biol Mol Vet, Cuiaba, MT, Brazil

[2] Univ Fed Mato Grosso UFMT, Hosp Vet HOVET, Fac Med Vet FAVET, Lab Patol Vet, Cuiaba, MT, Brazil

[3] Univ Fed Mato Grosso UFMT, Hosp Vet HOVET, Fac Med Vet FAVET, Lab Parasitol Vet & Doencas Parasitarias Anim Dom, Cuiaba, MT, Brazil

[4] Univ Fed Mato Grosso UFMT, Hosp Vet HOVET, Fac Med Vet FAVET, Setor Anim Silvestres, Cuiaba, MT, Brazil

[5] Univ Fed Mato Grosso UFMT, Hosp Vet HOVET, Fac Med Vet FAVET, Lab Leishmanioses, Cuiaba, MT, Brazil

来源：

REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA | 2021年 / 30卷 / 02期

关键词：

PCR; protozoan; phylogenetic analysis; zoonosis; Leishmaniasis; PCR; LEISHMANIA; BLOOD;

D O I：

10.1590/S1984-29612021041

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Trypanosomatids are uniflagellate protozoa belonging to the Trypanosomatidae family. The genera Trypanosoma and Leishmania are of paramount importance as they contain species that cause serious diseases, such as Chagas disease and Leishmaniasis, respectively. The objective of the present study was to identify trypanosomatids present in the whole blood of free-living and captive neotropical primates in Mato Grosso State, Midwest Brazil. Between 2017 and 2019, 38 blood samples were collected from seven different neotropical primate species in seven cities in the state. Through molecular techniques, including polymerase chain reaction (PCR) to amplify a fragment of the kinetoplast DNA (kDNA) and 18S ribosomal RNA (18S rRNA) gene, sequencing, and phylogenetic analysis, nine Leishmania spp. [seven L. infantum and two L. (Leishmania) amazonensis] and two Trypanosoma spp. (T. minasense and T. rangeli) were identified. This study contributes to understanding the occurrence and epidemiology of trypanosomatids in Mato Grosso State and the importance of neotropical primates as trypanosome hosts and possible infection sources for other animals and humans. Future identification of other blood pathogens in neotropical primates will assist in disease control and prevention strategies.

引用

页数：7

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