Efficient Delivery of Bioactive Antibodies into the Cytoplasm of Living Cells by Charge-Conversional Polyion Complex Micelles

被引：167

作者：

Lee, Yan ^{[2
]}

Ishii, Takehiko ^{[1
]}

Kim, Hyun Jin ^{[3
]}

Nishiyama, Nobuhiro ^{[4
,5
]}

Hayakawa, Yoshiyuki ^{[3
]}

Itaka, Keiji ^{[4
,5
]}

Kataoka, Kazunori ^{[1
,3
,4
,5
]}

机构：

[1] Univ Tokyo, Dept Bioengn, Bunkyo Ku, Tokyo 1138656, Japan

[2] Seoul Natl Univ, Dept Chem, Seoul 151742, South Korea

[3] Univ Tokyo, Dept Mat Engn, Tokyo 1138656, Japan

[4] Univ Tokyo, Ctr Dis Biol & Integrat Med, Grad Sch Med, Tokyo 1138656, Japan

[5] Univ Tokyo, Ctr Nanobio Integrat, Tokyo 1138656, Japan

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2010年 / 49卷 / 14期

基金：

日本科学技术振兴机构;

关键词：

antibodies; biological activity; charge conversion; drug delivery; micelles; GENE DELIVERY; POLYMER; POLYPLEX; PH; PROTEINS;

D O I：

10.1002/anie.200905264

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) Stand and deliver! Immunoglobulin G (IgG) can be delivered into the cytoplasm of living cells by charge-conversional modification followed by treatment with a cationic block copolymer to form polyion complex (PIC) micelles (see picture). The bioactivity of the IgC selectively recovers in the cell in a pH-dependent manner, thereby controlling the growth of human hepatoma cells through IgC binding to intracellular target molecules. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：2552 / 2555

页数：4

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