A Study on Hypoxia Susceptibility of Organ Tissues by Fluorescence Imaging with a Ratiometric Nitroreductase Probe

被引：57

作者：

Sarkar, Sourav ^{[1
]}

Lee, Hyori ^{[1
]}

Ryu, Hye Gun ^{[1
]}

Singha, Subhankar ^{[2
]}

Lee, Yeong Mi ^{[1
]}

Reo, Ye Jin ^{[1
]}

Jun, Yong Woong ^{[1
]}

Kim, Kyeong Hwan ^{[1
]}

Kim, Won Jong ^{[1
]}

Ahn, Kyo Han ^{[1
]}

机构：

[1] Pohang Univ Sci & Technol POSTECH, Dept Chem, Pohang 37673, Gyungbuk, South Korea

[2] JIS Univ, Inst Adv Studies & Res, Kolkata 700091, India

来源：

ACS SENSORS | 2021年 / 6卷 / 01期

基金：

新加坡国家研究基金会;

关键词：

nitroreductase; hypoxia; ratiometric probe; two-photon probe; hypoxia susceptibility; TUMOR HYPOXIA; OXYGENATION;

D O I：

10.1021/acssensors.0c01989

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Hypoxia, a condition of oxygen deficiency in tissues, features various diseases including solid tumor. Under hypoxia, several reductases such as nitroreductases are elevated. Based on this fact, we have investigated an indirect way to assess the hypoxia susceptibility of different organ tissues (mouse lung, heart, spleen, kidney, and liver) by detecting nitroreductase present within. Among the organs, the kidney showed a notable susceptibility to hypoxia, which was due to the renal medulla, not due to the renal cortex, as observed by ratiometric fluorescence imaging with a probe. The probe features ratiometric signaling, NIR-emitting, two-photon absorbing, and pH-insensitive emission properties, offering a practical tool for studying the nitroreductase activity and, furthermore, hypoxia-associated biological processes.

引用

页码：148 / 155

页数：8