Genome sequence of a carbapenemase-encoding Acinetobacter baumannii isolate of the sequence type 231 isolated from hospital wastewater in South Africa

Objectives: The resistome, virulome, mobilome and phylogenetic relationship of the Acinetobacter bau-mannii isolate FG121 depicting the multilocus sequence type (ST) 231 isolated from hospital effluent wa-ter in South Africa was determined using whole-genome sequence analysis. Method: A. baumannii FG121 was isolated on Leed Acinetobacter Medium (LAM) agar and the bacterial isolate was identified using the VITEK (R) 2 platform. Antibiotic susceptibility testing was performed using Kirby-Bauer Disk diffusion method. A whole genome sequencing library was constructed from DNA ex-tracted from the isolate using the Illumina Nextera XT library preparation kit and was sequenced using the Illumina NextSeq500 platform. Generated reads were de novo assembled using SpAdes v.3.9. The as-sembled contigs were annotated, and multilocus sequence type, antimicrobial resistance, and virulence genes were identified. Results: The resistome was consistent with the resistance phenotype of the isolate with resistance determinants for beta-lactams, aminoglycosides, and tetracycline (blaADC-25, blaOXA-23, blaOXA-51, blaNDM-1, aph[3']-VIa and tet[B]). Global phylogenomic analysis using BacWGSTdb revealed that the isolate be-longed to the multilocus sequence type ST-231, similar to previously reported isolates from South Africa, the United States, and related to the invasive KR3831 isolate identified from Oman in 2012, suggest -ing the isolate might be imported from abroad. Virulome analysis predicted both virulence and biofilm-determinants of A. baumannii, which may help to establish infections in adverse conditions. Conclusion: This is the first report on a carbapenemase-encoding A. baumannii ST-231 isolated from hos-pital effluent water. Our data will offer insight into the global phylogenetic, pathogenicity and distribution of A. baumannii in South Africa. (C) 2022 The Author(s). Published by Elsevier Ltd on behalf of International Society for Antimicrobial Chemotherapy