High-performance liquid chromatographic analysis of phlorotannins from the brown alga Fucus vesiculosus

被引：114

作者：

Koivikko, Riitta ^{[1
]}

Loponen, Jyrki

Pihlaja, Kalevi

Jormalainen, Veijo

机构：

[1] Univ Turku, Dept Biol, Sect Ecol, FIN-20014 Turku, Finland

[2] Univ Turku, Dept Chem, Lab Organ Chem & Chem Biol, FIN-20014 Turku, Finland

来源：

PHYTOCHEMICAL ANALYSIS | 2007年 / 18卷 / 04期

关键词：

NP-HPLC; antioxidant; oxidation; phenolic compounds;

D O I：

10.1002/pca.986

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Separating individual compounds by HPLC represents an effective method for the detection and quantification of phenolic compounds and has been widely utilised. However, phlorotannins are commonly quantified using colorimetric methods, as the total amount of the whole compound group. In the present paper the separation of a set of individual soluble phlorotannins from the phenolic crude extract of Fucus vesiculosus was achieved by HPLC with UV photodiode array detection. Different gradient programs for reversed- and normal-phase HPLC methods were developed and tested. Normal-phase (NP) conditions with a silica stationary phase and a mobile phase with a linear gradient of increasing polarity were found to separate 16 individual components of the phenolic extract. The suitability of the NP-HPLC method for mass spectrometric application was preliminarily tested. Sample preparation was found to be a critical step in the analysis owing to the rapid oxidation of phlorotannins; ascorbic acid was used as an antioxidant. Copyright (C) 2007 John Wiley & Sons, Ltd.

引用

页码：326 / 332

页数：7

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