Prospects for engineering dynamic CRISPR-Cas transcriptional circuits to improve bioproduction

被引：14

作者：

Fontana, Jason ^{[1
,2
]}

Voje, William E. ^{[1
,2
,3
]}

Zalatan, Jesse G. ^{[1
,2
,4
]}

Carothers, James M. ^{[1
,2
,3
]}

机构：

[1] Univ Washington, Mol Engn & Sci Inst, Seattle, WA 98195 USA

[2] Univ Washington, Ctr Synthet Biol, Seattle, WA 98195 USA

[3] Univ Washington, Dept Chem Engn, Seattle, WA 98195 USA

[4] Univ Washington, Dept Chem, Seattle, WA 98195 USA

来源：

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY | 2018年 / 45卷 / 07期

基金：

美国国家科学基金会;

关键词：

CRISPR-Cas; Transcriptional control; Metabolic engineering; Biosensors; Dynamic control; SEQUENCE-SPECIFIC CONTROL; SACCHAROMYCES-CEREVISIAE; ESCHERICHIA-COLI; GENE-EXPRESSION; GUIDE RNAS; INTERFERENCE CRISPRI; METABOLIC PATHWAYS; IN-VITRO; ACTIVATION; BACTERIA;

D O I：

10.1007/s10295-018-2039-z

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Dynamic control of gene expression is emerging as an important strategy for controlling flux in metabolic pathways and improving bioproduction of valuable compounds. Integrating dynamic genetic control tools with CRISPR-Cas transcriptional regulation could significantly improve our ability to fine-tune the expression of multiple endogenous and heterologous genes according to the state of the cell. In this mini-review, we combine an analysis of recent literature with examples from our own work to discuss the prospects and challenges of developing dynamically regulated CRISPR-Cas transcriptional control systems for applications in synthetic biology and metabolic engineering.

引用

页码：481 / 490

页数：10

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