A graphene-enhanced molecular beacon for homogeneous DNA detection

被引：207

作者：

Li, Fan ^{[1
]}

Huang, Yan ^{[2
]}

Yang, Qing ^{[1
]}

Zhong, Zentao ^{[2
]}

Li, Di ^{[1
]}

Wang, Lihua ^{[1
]}

Song, Shiping ^{[1
]}

Fan, Chunhai ^{[1
]}

机构：

[1] Chinese Acad Sci, Shanghai Inst Appl Phys, Phys Biol Lab, Shanghai 201800, Peoples R China

[2] Nanjing Agr Univ, Nanjing 213017, Jiangsu Prov, Peoples R China

来源：

NANOSCALE | 2010年 / 2卷 / 06期

关键词：

MESSENGER-RNA DETECTION; HYBRIDIZATION; OXIDE; PROBES; IMMOBILIZATION; FLUORESCE; KINETICS; MISMATCH;

D O I：

10.1039/b9nr00401g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In this work, we report the design of a novel graphene-based molecular beacon (MB) that could sensitively and selectively detect specific DNA sequences. The ability of water-soluble graphene oxide (GO) to differentiated hairpin and dsDNA offered a new approach to detect DNA. We found that the background fluorescence of MB was significantly suppressed in the presence of GO, which increased the signal-to-background ratio, hence the sensitivity. Moreover, the single-mismatch differentiation ability of hairpin DNA was maintained, leading to high selectivity of this new method.

引用

页码：1021 / 1026

页数：6

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