Possible role of nitric oxide (NO) in the regulation of gender related differences in stress induced anxiogenesis in rats

被引:17
作者
Chakraborti, Ayanabha [1 ]
Gulati, Kavita [1 ]
Ray, Arunabha [1 ]
机构
[1] Univ Delhi, Vallabhbhai Patel Chest Inst, Dept Pharmacol, Delhi 110007, India
来源
NITRIC OXIDE-BIOLOGY AND CHEMISTRY | 2014年 / 43卷
关键词
Stress; Gender difference; Anxiety; Nitric oxide; Estrogen; CEREBRAL BLOOD-VESSELS; DIMETHYLARGININE DIMETHYLAMINOHYDROLASE; ASYMMETRIC DIMETHYLARGININE; ESTROGEN; BRAIN; SYNTHASE; ADAPTATION; BEHAVIOR; EXPRESSION; ANXIETY;
D O I
10.1016/j.niox.2014.08.005
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Gender related differences in stress induced neurobehavioral disorders have been reported although the mechanisms involved are not yet clear. The present study investigated the role of nitric oxide, an important biomodulator in the sex related differences in stress induced anxiety like behavior in rats. Restraint stress (RS for 1 h) was used as the experimental stressor and the effects of NO modulators were assessed in the elevated plus maze (EPM) test in both male and female rats. No metabolites (NOx) and asymmetric dimethyl arginine (ADMA) were measured in brain homogenates of these rats for corroborative purposes. RS induced anxiogenesis in both male and female rats and such changes were greater in males as compared to females. The behavioral alterations were associated with enhanced levels of ADMA and reductions in levels of NOx in brain homogenates - the effects being greater in intensity in males as compared to females. Pretreatment with NO precursor L-arginine (500 mg/kg) reversed the RS induced behavioral and biochemical changes, while NO synthase inhibitor L-NAME (50 mg/kg) had opposite effects. Additionally, Formestane (50 mg/kg), an estrogen synthesis blocker, aggravated stress induced anxiogenesis with a corresponding increase in ADMA and decrease in NOx levels in the females. To our knowledge, this is the first report indicating the involvement of ADMA, an endogenous nitric oxide synthase inhibitor in stress induced neurobehavioral changes. Furthermore, it is also evident that nitric oxide and its interactions with estrogens play a crucial modulatory role in the differential anxiogenic response to stress among males and females. (c) 2014 Elsevier Inc. All rights reserved.
引用
收藏
页码:74 / 80
页数:7
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