The Amelioration of Pain-Related Behavior in Mice with Chronic Spinal Cord Injury Treated with Neural Stem/Progenitor Cell Transplantation Combined with Treadmill Training

被引：31

作者：

Tashiro, Syoichi ^{[1
]}

Nishimura, Soraya ^{[2
]}

Shinozaki, Munehisa ^{[3
]}

Takano, Morito ^{[2
]}

Konomi, Tsunehiko ^{[2
,4
]}

Tsuji, Osahiko ^{[2
]}

Nagoshi, Narihito ^{[2
]}

Toyama, Yoshiaki ^{[2
]}

Liu, Meigen ^{[1
]}

Okano, Hideyuki ^{[3
]}

Nakamura, Masaya ^{[2
]}

机构：

[1] Keio Univ, Sch Med, Dept Rehabil Med, Tokyo, Japan

[2] Keio Univ, Sch Med, Dept Orthopaed Surg, Tokyo, Japan

[3] Keio Univ, Sch Med, Dept Physiol, Tokyo, Japan

[4] Natl Hosp Org, Murayama Med Ctr, Dept Orthopaed Surg, Tokyo, Japan

来源：

JOURNAL OF NEUROTRAUMA | 2018年 / 35卷 / 21期

基金：

日本学术振兴会; 日本科学技术振兴机构;

关键词：

allodynia; exercise; neuropathic pain; regenerative medicine; rehabilitation; sensory function; CENTRAL NEUROPATHIC PAIN; NEUROTROPHIC FACTOR; STEM-CELLS; ASTROCYTE ACTIVATION; MECHANICAL ALLODYNIA; FUNCTIONAL RECOVERY; GROWTH-FACTOR; UP-REGULATION; MOUSE MODEL; CONTRIBUTES;

D O I：

10.1089/neu.2017.5537

中图分类号：

R4 [临床医学];

学科分类号：

1002 ; 100602 ;

摘要：

Progress in regenerative medicine is realizing the possibility of neural regeneration and functional recovery in spinal cord injury (SCI). Recently, rehabilitation has attracted much attention with respect to the synergistic promotion of functional recovery in combination with neural stem/progenitor cell (NS/PC) transplantation, even in the chronic refractory phase of SCI. Nevertheless, sensory disturbance is one of the most prominent sequelae, even though the effects of combination or single therapies have been investigated mostly in the context of motor recovery. To determine how combination therapy with treadmill training (TMT) and NS/PC transplantation affects the manifestation of thermal allodynia and tactile hyperalgesia in chronic phase SCI, four groups of SCI mice were used to assess pain-related behavior and histological changes: combined transplantation and TMT therapy, transplantation only, TMT only, and control groups. Thermal allodynia and coarse touch-pressure hyperalgesia exhibited significant recovery in the combined therapy group in comparison with controls, whereas there were no significant differences with fine touch-pressure hyperalgesia and motor function. Further investigation revealed fewer fibers remaining in the posterior funiculus, which contained the tracts associated with the two modalities showing less recovery; that is, touch-pressure hyperalgesia and motor function. A significant correlation was only observed between these two modalities. Although no remarkable histological recovery was found within the lesion epicenter, changes indicating amelioration of pain were observed in the lumbar enlargement of the combination therapy group. Our results suggest that amelioration of thermal allodynia and tactile hyperalgesia can be brought about by the additive effect of NS/PC transplantation and TMT. The degree of recovery seems dependent on the distribution of damage.

引用

页码：2561 / 2571

页数：11