UV-C irradiation delays the physiological changes of bell pepper fruit during storage

被引：32

作者：

Ma, Lili ^{[1
,2
]}

Wang, Qing ^{[1
]}

Li, Li ^{[3
]}

Grierson, Donald ^{[4
]}

Yuan, Shuzhi ^{[1
]}

Zheng, Shufang ^{[1
]}

Wang, Yunxiang ^{[5
]}

Wang, Baogang ^{[5
]}

Bai, Chunmei ^{[1
]}

Fu, Anzhen ^{[1
]}

Gao, Lipu ^{[1
]}

Zhu, Benzhong ^{[6
]}

Luo, Yunbo ^{[6
]}

Mu, Jianlou ^{[2
]}

Zuo, Jinhua ^{[1
]}

机构：

[1] Beijing Acad Agr & Forestry Sci, Key Lab Biol & Genet Improvement Hort Crops North, Key Lab Vegetable Postharvest Proc,Beijing Key La, Minist Agr,Key Lab Urban Agr North,Beijing Vegeta, Beijing 100097, Peoples R China

[2] Hebei Agr Univ, Coll Food Sci & Technol, Baoding 071001, Peoples R China

[3] Cornell Univ, Robert W Holley Ctr Agr & Hlth, USDA ARS, Ithaca, NY 14853 USA

[4] Univ Nottingham, Sch Biosci, Sutton Bonington Campus, Loughborough LE12 5RD, Leics, England

[5] Beijing Acad Agr & Forestry Sci, Beijing Acad Forestry & Pomol Sci, Beijing 100093, Peoples R China

[6] China Agr Univ, Coll Food Sci & Nutr Engn, Dept Food Biotechnol, Lab Postharvest Mol Biol Fruits & Vegetables, Beijing 100083, Peoples R China

来源：

POSTHARVEST BIOLOGY AND TECHNOLOGY | 2021年 / 180卷

基金：

芬兰科学院; 中国国家自然科学基金;

关键词：

Metabolome; Noncoding RNAs; Sweet pepper; Transcriptome; UV-C treatment; WALL-DEGRADING ENZYMES; ABSCISIC-ACID; ANTIOXIDANT CAPACITY; PROFILING ANALYSIS; GENE-EXPRESSION; NONCODING RNAS; ETHYLENE; IDENTIFICATION; METABOLISM; NETWORK;

D O I：

10.1016/j.postharvbio.2021.111506

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Bell pepper is a model for studying the ripening process of non-climacteric fleshy fruit, during which numerous physiological changes occur. UV-C irradiation is an abiotic stress, which has an important influence on plant growth, development and ripening. We explored the influences of UV-C irradiation on physiological changes in bell pepper fruit during postharvest storage by measuring sensory quality, firmness and mass loss, and the underlying changes at the molecular level by combined transcriptome and metabolome analysis. Effects were detected on transcripts for numerous critical enzymes involved in fruit pigment accumulation, fruit texture change, fruit flavor and aroma production. Moreover, transcripts for several key enzymes and transcription factors involved in plant hormone pathways and their corresponding noncoding RNAs were identified and differential expression profiles were also analyzed. Components of a competing endogenous RNAs (ceRNA) regulatory network changed in response to UV-C irradiation and the relationships between them was analyzed. Thirty-one differentially expressed metabolites were identified. Combined transcriptome and metabolome analysis showed that the genes and metabolites that responded to UV-C were mainly involved in flavonoid, phenylalanine, tyrosine, tryptophan and phenylpropanoid biosynthesis. This study provides insights into bell pepper fruit physiology and the effects of UV-C irradiation on postharvest quality and enhances our understanding of the underlying mechanism whereby UV-C delays postharvest physiological changes in bell pepper fruit.

引用

页数：12

共 89 条

[1] Characterisation of ethylene pathway components in non-climacteric capsicum[J]. Aizat, Wan M.;Able, Jason A.;Stangoulis, James C. R.;Able, Amanda J. BMC PLANT BIOLOGY, 2013
[2] Unravelling the complexity of microRNA-mediated gene regulation in black pepper (Piper nigrum L.) using high-throughput small RNA profiling[J]. Asha, Srinivasan;Sreekumar, Sweda;Soniya, E. V. PLANT CELL REPORTS, 2016(01)
[3] Genome-Wide Identification, Functional Analysis and Expression Profiling of the Aux/IAA Gene Family in Tomato[J]. Audran-Delalande, Corinne;Bassa, Carole;Mila, Isabelle;Regad, Farid;Zouine, Mohamed;Bouzayen, Mondher. PLANT AND CELL PHYSIOLOGY, 2012(04)
[4] Effects of solar ultraviolet radiation on terrestrial ecosystems. Patterns, mechanisms, and interactions with climate change[J]. Ballare, C. L.;Caldwell, M. M.;Flint, S. D.;Robinson, A.;Bornman, J. F. PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, 2011(02)
[5] Impact of UV-C irradiation on the cell wall-degrading enzymes during ripening of tomato (Lycopersicon esculentum L.) fruit[J]. Barka, EA;Kalantari, S;Makhlouf, J;Arul, J. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2000(03)
[6] Bintsis T, 2000, J SCI FOOD AGR, V80, P637, DOI 10.1002/(SICI)1097-0010(20000501)80:6<637::AID-JSFA603>3.0.CO
[7] 2-1
[8] CaGLK2 regulates natural variation of chlorophyll content and fruit color in pepper fruit[J]. Brand, Arnon;Borovsky, Yelena;Hill, Theresa;Rahman, Khalis Afnan Abdul;Bellalou, Aharon;Van Deynze, Allen;Paran, Ilan. THEORETICAL AND APPLIED GENETICS, 2014(10)
[9] Postharvest ultraviolet-C irradiation suppressed Psy 1 and Lcy-β-expression and altered color phenotype in tomato (Solanum lycopersicum) fruit[J]. Bu, Jianwen;Ni, Zhendan;Aisikaer, Guzhanuer;Jiang, Zhenhui;Khan, Zia Ullah;Mou, Wangshu;Ying, Tiejin. POSTHARVEST BIOLOGY AND TECHNOLOGY, 2014
[10] Postharvest UV-C irradiation inhibits the production of ethylene and the activity of cell wall-degrading enzymes during softening of tomato (Lycopersicon esculentum L.) fruit[J]. Bu, Jianwen;Yu, Yucong;Aisikaer, Guzhanuer;Ying, Tiejin. POSTHARVEST BIOLOGY AND TECHNOLOGY, 2013

← 1 2 3 4 5 6 7 8 9 →