Combined effect of hydrogen peroxide induced oxidative stress and IL-1α on IL-8 production in CaCo-2 cells (a human colon carcinoma cell line) and normal intestinal epithelial cells

被引:51
|
作者
Yamamoto, K [1 ]
Kushima, R
Kisaki, O
Fujiyama, Y
Okabe, H
机构
[1] Shiga Univ Med Sci, Dept Clin Lab Med, Otsu, Shiga 5202192, Japan
[2] Japan Clin Labs Inc, Kyoto, Japan
[3] Shiga Univ Med Sci, Dept Gastroenterol, Otsu, Shiga, Japan
关键词
oxidative stress; hydrogen peroxide; IL-8; IL-1; alpha;
D O I
10.1023/A:1023813710941
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Oxidative stress, IL-1alpha, and IL-8 are known to contribute to mucosal inflammation of the gastrointestinal tract. We examined the IL-8 response after brief exposure to hydrogen peroxide induced oxidative stress in CaCo-2 cells (a human colon carcinoma cell line) and in human intestinal epithelial cells. In addition, we examined whether exposure to oxidative stress, followed by IL-1alpha, could modulate IL-8 production. A transient up-regulation of IL-8 mRNA expression was observed after hydrogen peroxide treatment. Hydrogen peroxide induced oxidative stress was also observed to promote IL-8 secretion. Exposure to hydrogen peroxide, followed by IL-1alpha, enhanced IL-8 production over that achieved with IL-1alpha alone. Thus, oxidative stress and IL-1alpha were observed to cooperatively enhance IL-8 production.
引用
收藏
页码:123 / 128
页数:6
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