Cloning and characterization of 5′ upstream promoter region of rat WAP gene

被引：1
|
作者：
Jura, J [1 ]
Jura, J [1 ]
Murzyn, K
Wegrzyn, P
Zarebski, A
机构：
[1] Natl Res Inst Anim Prod, Dept Anim Reprod, PL-32083 Balice Krakow, Poland
[2] Jagiellonian Univ, Fac Biotechnol, Dept Cellular Biochem, Krakow, Poland
[3] Jagiellonian Univ, Fac Biotechnol, Dept Biophys, Krakow, Poland
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 2005年 / 1727卷 / 01期
关键词：
whey acidic protein gene; STAT5; rat WAP promoter; mammary gland;
D O I：
10.1016/j.bbaexp.2004.11.008
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Regulatory regions of genes encoding milk proteins are frequently used to produce in the mammary gland of transgenic animals a variety of pharmaceutically and medically important human proteins. One such example is the whey acidic protein (WAP) promoter region, identified so far in the genome of mouse, rat, rabbit, camel, pig, brushtail possum and Tammar wallaby. The aim of the present study was cloning and characterization of the 5' upstream promoter region of rat WAP gene. Using Genome Walking procedure, we cloned the region extending from -849 to -3671 bp. We have shown that there are two conserved regions highly similar to hypersensitive sites present in mouse and rabbit upstream region of WAP gene with binding sites for STAT5 transcription factor, essential for expression of WAP gene in mammary glands during lactation. We characterized dispersed and tandem repeats in the upstream region of rat WAP gen localized not far away from the translation initiation site. (C) 2004 Elsevier B.V All rights reserved.
引用
收藏
页码：58 / 64
页数：7
相关论文
共 50 条
  • [1] cDNA cloning and characterization of 5′ upstream promoter region of porcine mef2c gene
    Min Liu
    De Quan Xu
    Jian Peng
    Rong Zheng
    Fenge Li
    Si Wen Jiang
    Molecular Biology Reports, 2011, 38 : 4723 - 4730
  • [2] cDNA cloning and characterization of 5' upstream promoter region of porcine mef2c gene
    Liu, Min
    Xu, De Quan
    Peng, Jian
    Zheng, Rong
    Li, Fenge
    Jiang, Si Wen
    MOLECULAR BIOLOGY REPORTS, 2011, 38 (07) : 4723 - 4730
  • [3] Cloning and characterization of the promoter region of the rat taurine transporter (TauT) gene
    Han, XB
    Budreau, AM
    Chesney, RW
    TAURINE 4: TAURINE AND EXCITABLE TISSUES, 2000, 483 : 97 - 108
  • [4] Cloning and characterization of the promoter region of the rat taurine transporter gene.
    Han, X
    Budreau, AM
    Chesney, RW
    JOURNAL OF INVESTIGATIVE MEDICINE, 1998, 46 (01) : 44A - 44A
  • [5] Characterization of the 5′ upstream region of the Wilson disease gene and identification of a putative promoter region.
    Shah, AB
    Ross, BM
    Gilliam, TC
    AMERICAN JOURNAL OF HUMAN GENETICS, 1997, 61 (04) : A382 - A382
  • [6] Characterization, cloning and analysis of the promoter region of the SNAT2 gene of the rat
    Ortiz, Victor
    Aleman, Gabriela
    Escamilla, Martin
    Recillas-Targa, Felix
    Torres, Nimbe
    Tovar, Armando R.
    FASEB JOURNAL, 2008, 22
  • [7] Cloning and characterization of 5′-upstream region of human phospholipase C-β2 gene
    Yun, ES
    Lee, SJ
    Kim, MJ
    Ryu, SH
    Suh, PG
    EXPERIMENTAL AND MOLECULAR MEDICINE, 2001, 33 (02): : 76 - 82
  • [8] Cloning and characterization of 5'-upstream region of human phospholipase C-β2 gene
    Eun Sook Yun
    Seung-Jae Lee
    Myung Jong Kim
    Sung Ho Ryu
    Pann-Ghill Suh
    Experimental & Molecular Medicine, 2001, 33 : 76 - 82
  • [9] Cloning and characterization of rat dentin matrix protein 1 (DMP1) gene and its 5′-upstream region
    Thotakura, SR
    Karthikeyan, N
    Smith, T
    Liu, K
    George, A
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (14) : 10272 - 10277
  • [10] Cloning and Analysis of the Promoter Region of Rat uPA Gene
    Yan LIU
    JournalofReproductionandContraception, 2007, (01) : 1 - 10
12345