Genome-wide characterization and expression profiling of diacylglycerol acyltransferase genes from maize

被引：15

作者：

Yan, Bowei ^{[1
,2
]}

Xu, Xiaoxuan ^{[1
]}

Gu, Yingnan ^{[3
]}

Zhao, Ying ^{[1
]}

Zhao, Xunchao ^{[1
]}

He, Lin ^{[1
]}

Zhao, Changjiang ^{[1
]}

Li, Zuotong ^{[1
]}

Xu, Jingyu ^{[1
]}

机构：

[1] Heilongjiang Bayi Agr Univ, Coll Agr, Key Lab Modern Agr Cultivat & Crop Germplasm Impr, Daqing 163319, Heilongjiang, Peoples R China

[2] Heilongjiang Acad Land Reclamat Sci, Inst Comprehens Utilizat Agr & Anim Husb, Harbin 150000, Heilongjiang, Peoples R China

[3] Heilongjiang Acad Agr Sci, Remote Sensing Technol Ctr, Harbin 150086, Heilongjiang, Peoples R China

来源：

GENOME | 2018年 / 61卷 / 10期

关键词：

diacylglycerol acyltransferase (DGAT); triacylglycerol (TAG); gene expression; abiotic stress; maize (Zea mays L.); LIPID PRODUCTION; BRASSICA-NAPUS; OIL QUALITY; ACCUMULATION; ACID; IDENTIFICATION; BIOSYNTHESIS; METABOLISM; DATABASE; CLONING;

D O I：

10.1139/gen-2018-0029

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Diacylglycerol acyltransferase (DGAT) catalyzes the only rate-limiting step in the pathway of plant oil (TAG) biosynthesis and is involved in plant development. In this study, five DGAT family members were identified from maize genome database. Phylogenetic analysis classified the ZmDGATs into type-I, II, and III clusters. Conserved functional domain analysis revealed that the proteins encoded by ZmDGAT1 contained conserved MBOAT domains, while two ZmDGAT2-encoding proteins harbored LPLAT domains. qRT-PCR analysis showed that ZmDCAT genes exhibited very high relative expression in developing seeds, especially at the early stage of seed development. Under various abiotic stress conditions, differential responses of ZmDGAT genes were observed. An overall significant induction of ZmDGAT genes under cold stress in leaves and a quick and strong response to osmotic stresses in roots were highlighted. This study provides useful information for understanding the roles of DGATs in oil accumulation and stress responses in higher plants.

引用

页码：735 / 743

页数：9

共 42 条

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