Recognition of H3K9me1 by maize RNA-directed DNA methylation factor SHH2∞

被引：5

作者：

Wang, Yuhua ^{[1
,2
]}

Zhou, Xuelin ^{[1
,2
,3
]}

Luo, Jinyan ^{[1
,2
]}

Lv, Suhui ^{[4
]}

Liu, Rui ^{[4
]}

Du, Xuan ^{[4
]}

Jia, Bei ^{[1
,2
]}

Yuan, Fengtong ^{[1
,2
,3
]}

Zhang, Heng ^{[1
,2
]}

Du, Jiamu ^{[4
]}

机构：

[1] Chinese Acad Sci, Ctr Excellence Mol Plant Sci, Natl Key Lab Plant Mol Genet, Shanghai 201602, Peoples R China

[2] Chinese Acad Sci, Ctr Excellence Mol Plant Sci, Shanghai Ctr Plant Stress Biol, Shanghai 201602, Peoples R China

[3] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

[4] Southern Univ Sci & Technol, Inst Plant & Food Sci, Key Lab Mol Design Plant Cell Factory, Guangdong Higher Educ Inst,Sch Life Sci, Shenzhen 518055, Peoples R China

来源：

JOURNAL OF INTEGRATIVE PLANT BIOLOGY | 2021年 / 63卷 / 06期

基金：

中国国家自然科学基金;

关键词：

epigenetics; maize; RNA-directed DNA methylation; SAWADEE domain; SHH2; EPIGENETIC PATHWAY; POLYMERASE IV; COMPONENT; READOUT; L3MBTL1;

D O I：

10.1111/jipb.13103

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

RNA-directed DNA methylation (RdDM) is a plant-specific de novo DNA methylation pathway, which has extensive cross-talk with histone modifications. Here, we report that the maize RdDM regulator SAWADEE HOMEODOMAIN HOMOLOG 2 (SHH2) is an H3K9me1 reader. Our structural studies reveal that H3K9me1 recognition is achieved by recognition of the methyl group via a classic aromatic cage and hydrogen-bonding and salt-bridge interactions with the free protons of the mono-methyllysine. The di- and tri-methylation states disrupt the polar interactions, decreasing the binding affinity. Our study reveals a mono-methyllysine recognition mechanism which potentially links RdDM to H3K9me1 in maize.

引用

页码：1091 / 1096

页数：6

共 25 条

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