Micro-environmental influences on the fluorescence of tryptophan

被引：40

作者：

Sun, Feng ^{[1
]}

Zong, Wansong ^{[1
]}

Liu, Rutao ^{[1
]}

Chai, Jun ^{[1
]}

Liu, Ying ^{[1
]}

机构：

[1] Shandong Univ, Sch Environm Sci & Engn, Jinan 250100, Shandong, Peoples R China

来源：

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2010年 / 76卷 / 02期

关键词：

Tryptophan; Fluorescence spectroscopy; Micro-environment factors; CHEMICAL-MODIFICATION; AMINO-ACIDS; RESIDUES; SPECTROSCOPY; SPECTRA; MUTANT; LECTIN; BSA;

D O I：

10.1016/j.saa.2010.03.002

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

The fluorescence characteristics of protein molecules are mainly due to their tryptophan (Trp), tyrosine (Tyr) and phenylalanine (Phe) residues, among which tryptophan is the most important. Studying the influence of the micro-environment on tryptophan fluorescence can give us direct and convincing evidence for changes of protein structure and function. In this paper, fluorescence spectroscopy was used to evaluate the changes of tryptophan fluorescence under a variety of micro-environmental conditions (temperature, pH, polarity, presence of surfactants and oxidants) and the mechanisms responsible. This study not only presents more direct evidence to explain how and why the protein fluorescence spectra change, but also provides a new method for analyzing the effect of environmental changes on protein function. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：142 / 145

页数：4

共 22 条

[1] Fluorescence spectroscopy as a tool to investigate protein interactions [J].

Brown, MP ;

Royer, C .

CURRENT OPINION IN BIOTECHNOLOGY, 1997, 8 (01) :45-49

[2] A study of the Franck-Condon principle [J].

Coolidge, AS ;

James, HM ;

Present, RD .

JOURNAL OF CHEMICAL PHYSICS, 1936, 4 (03) :193-211

[3]

Dai Lei, 2008, Sichuan Daxue Xuebao (Ziran Kexueban), V45, P1519

[4] Fluorescence and folding properties of Tyr mutant tryptophan synthase α-subunits from Escherichia coli [J].

Jeong, JK ;

Shin, HJ ;

Kim, JW ;

Lee, CH ;

Kim, HD ;

Lim, WK .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2003, 300 (01) :29-35

[5] Long-range effects on dynamics in a temperature-sensitive mutant of trp repressor [J].

Jin, LH ;

Fukayama, JW ;

Pelczer, I ;

Carey, J .

JOURNAL OF MOLECULAR BIOLOGY, 1999, 285 (01) :361-378

[6] Fluorescence quenching, time-resolved fluorescence and chemical modification studies on the tryptophan residues of snake gourd (Trichosanthes anguina) seed lectin [J].

Komath, SS ;

Swamy, MJ .

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 1999, 50 (2-3) :108-118

[7] Fluorescence self-quenching and the quenching mechanism of magnolol [J].

Li Hong-Mei ;

Chen Ya-Dong ;

Yan Zheng-Yu ;

Hu Yu-Zhu .

ACTA PHYSICO-CHIMICA SINICA, 2007, 23 (09) :1454-1458

[8]

[梁彦秋 LIANG Yan-qiu], 2009, [化学研究与应用, Chemical Research and Application], V21, P155

[9] Evaluation on the toxicity of nanoAg to bovine serum albumin [J].

Liu, Rutao ;

Sun, Feng ;

Zhang, Lijun ;

Zong, Wansong ;

Zhao, Xingchen ;

Wang, Li ;

Wu, Ruolin ;

Hao, Xiaopeng .

SCIENCE OF THE TOTAL ENVIRONMENT, 2009, 407 (13) :4184-4188

[10]

Liu S. F., 1997, J HARBIN COMMERCIAL, V13, P46

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