Estrogen activation of cyclic adenosine 5′-monophosphate response element-mediated transcription requires the extracellularly regulated kinase/mitogen-activated protein kinase pathway

被引:136
作者
Wade, CNB
Dorsa, DM
机构
[1] Oregon Hlth & Sci Univ, Dept Physiol & Pharmacol, Portland, OR 97201 USA
[2] Univ Washington, Dept Pharmacol, Seattle, WA 98195 USA
关键词
D O I
10.1210/en.2002-220899
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The ability of estrogen to rapidly initiate a variety of signal transduction cascades is increasingly recognized as playing an important role in a number of tissue-specific transcriptional actions of the hormone. In vivo, estrogen rapidly elicits phosphorylation of cAMP response element-binding protein (CREB). We have previously shown that both ERalpha and ERbeta are capable of activating the MAPK pathway in response to a low dose of 17beta-estradiol. In the present study, the ability of estrogen to act through both ERalpha and ERbeta to increase CREB phosphorylation was evaluated in an immortalized hippocampal cell line stably expressing either receptor. Estrogen treatment promoted rapid CREB phosphorylation, reaching a maximum by 15 min. This activation is completely blocked by the antiestrogen ICI 182,780, suggesting an estrogen receptor-dependent mechanism. The addition of the mitogen/ERK kinase-1 inhibitor, PD98059, also blocked the ability of estrogen to signal to CREB phosphorylation. Estrogen also caused an increase in p90Rsk activity, a critical mediator of MAPK effects. Surprisingly, blockade of the protein kinase A pathway in cells treated with estrogen did not affect estrogen-mediated CREB phosphorylation. Thus, MAPK and p90Rsk appear to be the primary mediators of estrogen-induced gene transcription through ERalpha and ERbeta.
引用
收藏
页码:832 / 838
页数:7
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[41]   Redox regulation of the mitogen-activated protein kinase pathway during lymphocyte activation [J].
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Coderre, L .
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Das, S ;
Maizels, ET ;
DeManno, D ;
StClair, E ;
Adam, SA ;
HunzickerDunn, M .
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[46]   Pancreatic stellate cell activation by ethanol and acetaldehyde: Is it mediated by the mitogen-activated protein kinase signaling pathway? [J].
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[47]   ACTIVATION OF MITOGEN-ACTIVATED PROTEIN-KINASE EXTRACELLULAR SIGNAL-REGULATED KINASE KINASE BY G-PROTEIN AND TYROSINE KINASE ONCOPROTEINS [J].
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[48]   ACTIVATION OF THE ESTROGEN-RECEPTOR THROUGH PHOSPHORYLATION BY MITOGEN-ACTIVATED PROTEIN-KINASE [J].
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ENDOH, H ;
MASUHIRO, Y ;
KITAMOTO, T ;
UCHIYAMA, S ;
SASAKI, H ;
MASUSHIGE, S ;
GOTOH, Y ;
NISHIDA, E ;
KAWASHIMA, H ;
METZGER, D ;
CHAMBON, P .
SCIENCE, 1995, 270 (5241) :1491-1494
[49]   The cyclic adenosine monophosphate-dependent protein kinase (PKA) is required for the sustained activation of mitogen-activated kinases and gene expression by nerve growth factor [J].
Yao, H ;
York, RD ;
Misra-Press, A ;
Carr, DW ;
Stork, PJS .
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[50]   Activation of mitogen-activated protein kinase kinase (MEK)/extracellular signal-regulated kinase (ERK) signaling pathway is involved in myeloid lineage commitment [J].
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