Generalized polymer effective charge measurement by capillary isotachophoresis

被引：12

作者：

Chamieh, Joseph ^{[1
]}

Koval, Dusan ^{[2
]}

Besson, Adeline ^{[1
]}

Kasicka, Vaclav ^{[2
]}

Cottet, Herve ^{[1
]}

机构：

[1] Univ Montpellier 2, Univ Montpellier 1, Inst Biomol Max Mousseron, UMR CNRS 5247, F-34095 Montpellier 5, France

[2] Acad Sci Czech Republ, Inst Organ Chem & Biochem, Vvi, CR-16610 Prague 6, Czech Republic

来源：

JOURNAL OF CHROMATOGRAPHY A | 2014年 / 1370卷

关键词：

Polymer effective charge; Polyelectrolyte; Isotachophoresis; Counter-ion condensation; Capillary electrophoresis; ELECTROPHORETIC MOBILITY; COUNTERION CONDENSATION; POLYELECTROLYTE SOLUTIONS; NET CHARGE; LIMITING LAWS; UV DETECTION; SIZE; PEPTIDES; CONDUCTIVITY; ELECTROLYTE;

D O I：

10.1016/j.chroma.2014.10.025

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this work, we have generalized the use of capillary isotachophoresis as a universal method for determination of effective charge of anionic and cationic (co)polymers on ordinary capillary electrophoresis instruments. This method is applicable to a broad range of strong or weak polyelectrolytes with good repeatability. Experimental parameters (components and concentrations of leading and terminating electrolytes, capillary diameters, constant electric current intensity) were optimized for implementation in 100 mu m i.d. capillaries for both polyanions and polycations. Determined values of polymer effective charge were in a very good agreement with those obtained by capillary electrophoresis with indirect UV detection. Uncertainty of the effective charge measurement using isotachophoresis was addressed and estimated to be similar to 5-10% for solutes with mobilities in the 20-50 x 10(-9) m(2) V-1 s(-1) range. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：255 / 262

页数：8

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