Understanding DNA organization, damage, and repair with super-resolution fluorescence microscopy

被引：14
作者：
Miriklis, Esther L. [1 ]
Rozario, Ashley M. [1 ]
Rothenberg, Eli [2 ]
Bell, Toby D. M. [1 ]
Whelan, Donna R. [3 ]
机构：
[1] Monash Univ, Sch Chem, Clayton, Vic, Australia
[2] NYU, Sch Med, Perlmutter Canc Ctr, Dept Biochem & Mol Pharmacol, New York, NY USA
[3] La Trobe Univ, La Trobe Inst Mol Sci, Dept Pharm & Biomed Sci, Bendigo, Vic, Australia
来源：
METHODS AND APPLICATIONS IN FLUORESCENCE | 2021年 / 9卷 / 03期
基金：
澳大利亚研究理事会;
关键词：
DNA damage; chromatin; super resolution microscopy; DNA structure; genomic integrity; OPTICAL RECONSTRUCTION MICROSCOPY; SUBDIFFRACTION-RESOLUTION; HOMOLOGOUS RECOMBINATION; LOCALIZATION MICROSCOPY; BREAK REPAIR; CHROMATIN; VISUALIZATION; REPLICATION; LIMIT; TRANSCRIPTION;
D O I：
10.1088/2050-6120/abf239
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Super-resolution microscopy (SRM) comprises a suite of techniques well-suited to probing the nanoscale landscape of genomic function and dysfunction. Offering the specificity and sensitivity that has made conventional fluorescence microscopy a cornerstone technique of biological research, SRM allows for spatial resolutions as good as 10 nanometers. Moreover, single molecule localization microscopies (SMLMs) enable examination of individual molecular targets and nanofoci allowing for the characterization of subpopulations within a single cell. This review describes how key advances in both SRM techniques and sample preparation have enabled unprecedented insights into DNA structure and function, and highlights many of these new discoveries. Ongoing development and application of these novel, highly interdisciplinary SRM assays will continue to expand the toolbox available for research into the nanoscale genomic landscape.
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收藏
页数：21
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