Residual Negative Pressure in Vacuum Tubes Might Increase the Risk of Spurious Hemolysis

被引：1

作者：

Xiao, Tong-Tong ^{[1
]}

Zhang, Qiao-Xin ^{[1
]}

Hu, Jing ^{[1
]}

Ouyang, Hui-Zhen ^{[1
]}

Cai, Ying-Mu ^{[1
]}

机构：

[1] Shantou Univ, Dept Clin Lab, Med Coll, Affiliated Hosp 1, Shantou 515000, Peoples R China

来源：

CLINICAL LABORATORY | 2017年 / 63卷 / 5-6期

关键词：

vacuum tubes; hemolysis; pre-analytical; SAMPLES DRAWN; EMERGENCY; ERRORS;

D O I：

10.7754/Clin.Lab.2017.161125

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Background: We planned a study to establish whether spurious hemolysis may occur when negative pressure remains in vacuum tubes. Methods: Four tubes with different vacuum levels (-54, -65, -74, and -86 kPa) were used to examine blood drawn from one healthy volunteer; the tubes were allowed to stand for different times (1, 2, 3, and 4 hours). The plasma was separated and immediately tested for free hemoglobin (FHb). Thirty patients were enrolled in a verification experiment. Results: The degree of hemolysis observed was greater when the remaining negative pressure was higher. Significant differences were recorded in the verification experiment. Conclusions: The results suggest that residual negative pressure might increase the risk of spurious hemolysis.

引用

页码：1009 / 1013

页数：5

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