Protein A chromatography for antibody purification

被引：385
|
作者：
Hober, Sophia
Nord, Karin
Linhult, Martin
机构：
[1] Octapharma AB, S-11275 Stockholm, Sweden
[2] Affibody AB, Bromma, Sweden
[3] Royal Inst Technol, Stockholm, Sweden
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2007年 / 848卷 / 01期
关键词：
affinity chromatography; cleaning-in-place (CIP); deamidation; protein A; protein engineering; stabilization;
D O I：
10.1016/j.jchromb.2006.09.030
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Staphylococcal protein A (SPA) is one of the first discovered immunoglobulin binding molecules and has been extensively studied during the past decades. Due to its affinity to immunoglobulins, SPA has found widespread use as a tool in the detection and purification of antibodies and the molecule has been further developed to one of the most employed affinity purification systems. Interestingly, a minimized SPA derivative has been constructed and a domain originating from SPA has been improved to withstand the harsh environment employed in industrial purifications. This review will focus on the development of different affinity molecules and matrices for usage in antibody purification. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码：40 / 47
页数：8
相关论文
共 50 条
  • [21] Protein A chromatography: Challenges and progress in the purification of monoclonal antibodies
    Mayela Ramos-de-la-Pena, Ana
    Gonzalez-Valdez, Jose
    Aguilar, Oscar
    JOURNAL OF SEPARATION SCIENCE, 2019, 42 (09) : 1816 - 1827
  • [22] Optimizing high throughput antibody purification by using continuous chromatography media
    Butche, Rebecca E.
    Martin-Rousset, Genevieve
    Bradfor, Rebecca A.
    Teste, Andrea
    Owczarek, Catherine
    Hardy, Matthew P.
    Chen, Chao-Guang
    Sansome, Georgina
    Fabri, Louis J.
    Schmidt, Peter M.
    PROTEIN EXPRESSION AND PURIFICATION, 2019, 159 : 75 - 82
  • [23] Purification of Porcine Circovirus Type 2 Using an Affinity Chromatography Based on a Neutralizing Monoclonal Antibody against Viral Capsid Protein
    Bian, Haiqiao
    Yu, Chong
    Wei, Yanwu
    Feng, Li
    Liu, Changming
    Huang, Liping
    PATHOGENS, 2021, 10 (12):
  • [24] Studies on Purification of Polyclonal Antibody Against Soybean Globulin on Immunoaffinity Chromatography Column
    Yao L.
    Xi J.
    Chen H.
    Chen Y.
    Pi J.
    Li P.
    Journal of Chinese Institute of Food Science and Technology, 2021, 21 (06) : 296 - 300
  • [25] Performance of Protein-A-Based Affinity Membranes for Antibody Purification
    Uzun, Lokman
    Turkmen, Deniz
    Karakoc, Veyis
    Yavuz, Handan
    Denizli, Adil
    JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, 2011, 22 (17) : 2325 - 2341
  • [26] Affinity chromatography system for parallel purification of recombinant protein samples
    Gottstein, C
    Forde, R
    PROTEIN ENGINEERING, 2002, 15 (10): : 775 - 777
  • [27] Structural evaluation of an alternative Protein A biomimetic ligand for antibody purification
    Barroso, Telma
    Branco, Ricardo J. F.
    Aguiar-Ricardo, Ana
    Roque, Ana C. A.
    JOURNAL OF COMPUTER-AIDED MOLECULAR DESIGN, 2014, 28 (01) : 25 - 34
  • [28] Structural evaluation of an alternative Protein A biomimetic ligand for antibody purification
    Telma Barroso
    Ricardo J. F. Branco
    Ana Aguiar-Ricardo
    Ana C. A. Roque
    Journal of Computer-Aided Molecular Design, 2014, 28 : 25 - 34
  • [29] Thiacarbocyanine as ligand in dye-affinity chromatography for protein purification
    Boto, R. E. F.
    Almeida, P.
    Queiroz, J. A.
    BIOMEDICAL CHROMATOGRAPHY, 2008, 22 (03) : 278 - 288
  • [30] Aptamer affinity chromatography: combinatorial chemistry applied to protein purification
    Romig, TS
    Bell, C
    Drolet, DW
    JOURNAL OF CHROMATOGRAPHY B, 1999, 731 (02): : 275 - 284
12345