Protein A chromatography for antibody purification

被引：392
作者：
Hober, Sophia
Nord, Karin
Linhult, Martin
机构：
[1] Octapharma AB, S-11275 Stockholm, Sweden
[2] Affibody AB, Bromma, Sweden
[3] Royal Inst Technol, Stockholm, Sweden
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2007年 / 848卷 / 01期
关键词：
affinity chromatography; cleaning-in-place (CIP); deamidation; protein A; protein engineering; stabilization;
D O I：
10.1016/j.jchromb.2006.09.030
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Staphylococcal protein A (SPA) is one of the first discovered immunoglobulin binding molecules and has been extensively studied during the past decades. Due to its affinity to immunoglobulins, SPA has found widespread use as a tool in the detection and purification of antibodies and the molecule has been further developed to one of the most employed affinity purification systems. Interestingly, a minimized SPA derivative has been constructed and a domain originating from SPA has been improved to withstand the harsh environment employed in industrial purifications. This review will focus on the development of different affinity molecules and matrices for usage in antibody purification. (c) 2006 Elsevier B.V. All rights reserved.
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收藏
页码：40 / 47
页数：8
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